摘要
目的观察种植体周围炎动物模型建立前、后IFN-γ、IL-10的表达。方法选取5只beagle犬,每只犬拔除前磨牙,拔牙3个月后,全麻下植入穿龈式纯钛种植体。植入3个月后,在种植体颈部缠绕结扎丝3个月,骨缺损40%以上作为成功建立种植体周围炎模型的评价标准。模型建立前、后取龈沟液,ELISA法测定模型建立前、后IFN-γ、IL-10的含量,SPSS统计。结果种植体周围炎龈沟液中IFN-γ均值为6.64 ng/mL,对照组均值为2.22 ng/mL(P=0.01);IL-10均值为5.78ng/mL,对照组为4.78 ng/mL(P=0.54)。结论种植体周围炎龈沟液中IFN-γ有显著性升高,IL-10升高,但无统计学意义。
Objective To investigate the expression of IFN-γ and IL-10 before and after periimplantitis animal models were built.Methods Select 5 beagle dogs,extract premolars in each dog.3 months after extraction,gingiva perforating pure titanium implants were implanted under general anesthesia.3 months after implantation,ligature wires were used to bind the necks of implants for 3 months,and bone defect more than 40% reached the evaluation standard.Before and after peri-implantitis models were built,get the gingival crevicular fluid.Contents of IFN-γ、IL-10 were evaluated by enzyme-linked immunosorbent assay before and after peri-implantitis models were built.SPSS was used for statistical analysis.Results The mean value of IFN-γ in PICF was 6.64 ng / mL,while that in controll group was 2.22 ng / mL（P = 0.01）.The mean value of IL-10 in PICF was 5.78 ng / mL,while that in the controll group was 4.78 ng / mL（P = 0.54）.Conclusions Level of IFN-γ increases significantly in PICF,while IL-10 increases with no statistical significence in periimplantitis.
出处
《口腔医学》
CAS
2013年第7期450-452,共3页
Stomatology
基金
2012年浙江省教育厅科研资助项目(项目编号Y201225166)
