摘要
目的研究凋亡素2配体（Apo-2 ligand，Apo2L），又称肿瘤坏死因子相关凋亡诱导配体（tumornecrosisfactor-relatedapoptosisinducingligand，TRAIL）对体外培养食管癌细胞株Eca-109放射敏感性的影响。方法MTT法检测TRAIL对Eca-109细胞的毒性；克隆形成实验检测TRAIL对Eca-109细胞的放射增敏作用；流式细胞仪（FCM）技术分析TRAIL对凋亡率及细胞周期的影响。结果100～800μg／mlTRAIL随浓度升高对Eca-109细胞的增殖抑制率增大，药物浓度与细胞抑制率正相关（r=0．981，P〈0．01），50％抑制浓度（IC50）为637μg／ml；200μg／mlTRAIL能增加Eca-109细胞的放射敏感性，放射增敏比为1．219；不同剂量（0—8Gy）照射后，给药组的凋亡率均高于单照射组（F=16．97、76．65、92．86、209．66，P〈0．05），给药组G2／M期细胞比例较单照射组增加（F=9．40、84．99、87．61、2025．85，P〈0．05）。结论TRAIL可以抑制Eca-109细胞的生长，诱导细胞凋亡和G2／M期阻滞，后者可能与TRAIL的放射增敏机制有关。
Objective To study the radiosensitive effect of Apo-2 ligand （Apo2L）, known as tumor necrosis factor-related apoptosis inducing ligand （TRAIL） , on esophageal cancer cell line-Eca109 in vitro. Methods MTT assay and clonogenic assay were performed to evaluate the eytotoxicity and radiosensitization of TRAIL, respectively. Cell apoptosis and cell cycle distribution were measured by flow cytometry （FCM）. Results TRAIL inhibited cell growth in a dose-dependent manner and its 50% inhibition concentration（IC50） was 637μg/ml. The concentration 200μg/ml TRAIL could enhance cell radiosensitivity with a SER of 1. 219. After 2, 4, 6, 8 Gy X-ray irradiation, the incidence of apoptosis in TRAIL-treated cells was higher than that of irradiation alone groups（ F = 16. 97, 76.65, 92.86, 209.66, P 〈0.05）. The percentage of cells in G2/M phase of TRAIL-treated groups was also higher than that of irradiation alone groups （ F = 9.40, 84.99, 87.61, 2025.85, P 〈 0.05 ）. Conclusions TRAIL could inhibit cell growth, induce apoptosis and G2/M arrest, and had radiosensitization effect on Eea-109 cells.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2013年第3期278-281,共4页
Chinese Journal of Radiological Medicine and Protection
