摘要
目的:建立适合川东北地区表面健康人群抗线粒体抗体M2(AMA-M2)的参考范围。方法:体格检查和相关的实验室检查均无异常的表面健康人群共120例,按性别和年龄分组,采用Alegria全自动酶联免疫分析仪和配套的检测试剂来检测其血清AMA-M2浓度。结果:AMA-M2检测系统的批内和天间的检测精密度良好,其他性能参数符合临床使用要求。表面健康人群女性组血清AMA-M2浓度为1.90(1.70～4.50)IU/mL,明显高于男性组的1.85(1.60～2.70)IU/mL(Z=-2.804,P=0.005);而<50岁年龄组和≥50岁年龄组血清AMA-M2检测结果之间无统计学差异(Z=-0.317,P=0.751)。血清AMA-M2参考范围在女性组为0～4.45 IU/mL,男性组为0～2.50 IU/mL。结论:AMA-M2参考范围应按性别分别设定,建立一个适合本地区人群的AMA-M2参考范围具有重要的临床应用价值。
Objective：To establish the reference range of M2 antimitochondrial antibody（ AMA-M2 ） in Northeast of Sichuan appar- ently healthy individuals. Methods： The automated Alegria enzyme-linked immunosorbent assay system was applied to evaluate the concentration of antimitochondrial antibody in 120 apparently healthy individuals. Results： The automated Alegria enzyme-linked im- munosorbent assay system has good analytical performance. Within the apparently healthy individuals, the concentration of AMA-M2 in female [ 1.90 （ 1.70 - 4.50 ） IU/mL ] was higher than male [ 1. 85 （ 1.60- 2.70 ） IU/mL ] （ Z = - 2. 804, P = 0. 005 ）, and there was no significant difference between 〈 50 year old group and≥50 year old group. The reference range of AMA-M2 was 0 -4.45 IU/mL for female and 0 - 2.50 IU/mL for male. Conclusion： The reference range of AMA-M2 should be established with gender. The laboratory should establish the reference range of AMA-M2 to suit the local individuals for the important value of clinical application.
出处
《川北医学院学报》
CAS
2013年第4期310-313,共4页
Journal of North Sichuan Medical College
基金
国家"十一五"科技支撑课题(2008BA159B02)
