摘要
目的:研究北五味子藤茎总三萜化合物(TFSCBR)对小鼠急性酒精性肝损伤的保护作用。方法:采用56°北京红星二锅头白酒建立急性酒精肝损伤模型。将60只昆明小鼠随机分为6组,每组10只,分别为正常对照组、模型对照组及TFSCBR高、中、低剂量组和齐墩果酸阳性对照组。每天灌胃1次,TFSCBR高、中、低剂量分别为50、30、10mg/(kg.d),齐墩果酸阳性对照组剂量为30mg/(kg.d),正常对照组、模型对照组灌胃等量生理盐水,实验4周后,通过脏器质量和体质量计算肝脏指数,测定小鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)的含量,测定肝组织中过氧化脂质降解产物丙二醛(MDA)、还原型谷胱甘肽(GSH)和超氧化物歧化酶(SOD)含量。结果:中剂量的TFSCBR能显著降低酒精性肝损伤所引起的ALT、AST活性,抑制MDA含量的升高(P<0.01),有效地拮抗肝脏中SOD的活性与GSH含量的降低(P<0.01)。肝脏病理学改变减轻或逆转。结论:TFSCBR中剂量对酒精性肝损伤具有保护功能。
Objective： To explore the protective effect of triterpene compounds from Schisandra chinensis （Turcz） Baill rattan （TFSCBR） against acute alcohol liver injury in mice. Methods： Acute alcoholic liver injury model was induced by 56% Beijing Red Star Erguotou liquor. Totally 60 Kunming mice were randomly divided into 6 groups of 10 mice each： normal group, alcohol model group, oleanolic acid （positive control, 30 mg/（kg d）） group, and triterpenoid low, middle and high dose （10, 30 mg/（kg d） and 50 mg/（kg d）） groups. The normal group and alcohol model group were administered with an equal volume of normal saline. After 4 weeks of administration, the levels of serum alanine transaminase （ALT）, aspartate transaminase （AST） and liver malondialdehyde （MDA）, glutathione （GSH） and super oxid edismutase （SOD） were assayed. Results： TFSCBR treatment at the dose of 30 mg/（kg d） significantly reduced the elevation of AST, ALT and MDA （P 〈 0.01）, and significantly inhibited the decrease of SOD activity and GSH level （P 〈 0.01） in mice suffering from liver injury. Furthermore, hepatic pathological changes were attenuated or even reversed. Conclusion： TFSCBR treatment at the dose of 30 mg/（kg d） has a significant hepatoprotective effect against alcohol-induced liver injury in mice.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第15期228-231,共4页
Food Science
基金
国家自然科学基金青年科学基金项目(31201325)
辽宁省教育厅科学研究一般项目(L2011109)
关键词
北五味子总三萜
小鼠
酒精性肝损伤
triterpenoid from Schisandra chinensis （Turcz） Baill rattan： mouse
alcohol-induced liver injury
