摘要
目的制备叶酸羟丙基-β-环糊精包合物,以期提高叶酸的溶解性。方法采用溶液搅拌法制备叶酸羟丙基-β-环糊精包合物,利用核磁共振氢谱1H-NMR、红外光谱、圆二色谱和差示量热扫描对包合物进行表征,运用紫外-可见吸收光谱建立标准曲线方程对包合物中的叶酸进行定量。结果物理混合物图谱与包合物图谱存在显著差别。结论通过与羟丙基-β-环糊精(hydroxypropyl-β-cyclodextrin,HP-β-CD)形成包合物,叶酸的溶解性得到显著提高;叶酸被羟丙基-β-环糊精包合后呈现出新的理化特征,表明形成叶酸羟丙基-β-环糊精包合物。
Objective To prepare the 2 - hydroxypropyl - β - eyclodextrin folate acid inclusion compound, expecting to improve the solubility of folate acid. Methods Solution stirring method was utilized to prepare the 2 - hydroxypropyl - β - cyclodextrin folate acid inclusion complexes. Obtained inclusion complexes were qualified by 1H- NMR, infrared spectrometry, circular dichroism spectroscopy and DSC, and quantified by standard equation set up by Ultraviolet - Visible Absorption Spectroscopy. Results The analysis by nuclear magnetic resonance spectroscopy, infrared spectrometry, circular dichroism spectroscopy and differential scanning calorimetry showed an obvious difference between the inclusion complexes and folate acid. The standard curve equation calculation indicated a great increase in the solubility of folate acid in water. Conclusion Hydroxypropyl - β - eyelodextrin could obviously increase the solubility of folate acid. The inclusion complexes showed some new physical characteristics and those results obtained demonstrated the inclusion complexes were formed.
出处
《医学研究杂志》
2013年第8期98-101,共4页
Journal of Medical Research
关键词
主分子
客分子
手性分子
差示量热扫描
康顿效应
非极性场
包合物
Host molecule
Guest molecule
Chiral molecule
Differential scanning calorimeter
Cotton effect
Nonpolar field
Inclusion complexes
