摘要
目的构建蛋氨酸酶基因重组腺病毒载体.方法运用Cre-LoxP重组酶系统,通过DNA重组技术,将L-蛋氨酸-γ裂解酶基因(L-Methioneγ-Lyase Gene)与供体载体pDNR-CMV重组,获得含有Metase重组基因的供体载体pDNR-Metase-CMV.将pDNR-Metase-CMV片段与真核腺病毒载体PLP-Ade-X-CMV进行重组,获得pLP-Ade-Metase-CMV重组分子.结果获得了重组腺病毒载体pLP-Ade-Metase-CMV,并证实该重组腺病毒载体中有Metase Gene.结论采用LoxP/Cre体外重组法成功地构建了含蛋氨酸酶基因的真核表达重组腺病毒载体.
Objective To construct L-methionine γ-lyase gene recombinant adenovirus vector. Methods Using Cre-LoxP recombination system and recombinant DNA technology, L-methionine γ-lyase Gene was con- nected to the pDNR-CMV donor vector, and the pDNR-Metase-CMV recombinant vector containing 1V[etase was generated. Then, the eukaryotic adenovirus vector PLP-Ade-Metase-CMV was constructed from recombinate pDNR-Metase-CMV fragment and eukaryotic vector PLP-Ade-X-CMV in vitro. Results The recombinant aden- ovirus vector was obtained, and the Metase gene was confirmed to be contained. Conclusion The recombinant adenovirus vector containing L-methionine γ -lyase gene could be constructed by using LoxP/Cre in vitro recombi- nation.
出处
《昆明医科大学学报》
CAS
2013年第6期9-11,20,共4页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(307660287)
云南省科技厅重点基金资助项目(2009CC022)
关键词
肿瘤
蛋氨酸酶
重组腺病毒
Tumour
L-Methionine γ-lyase gene
Recombinant adenovirus
