期刊文献+

端粒保护蛋白1在不同证型获得性再生障碍性贫血患者中的表达及相关性研究 被引量:2

POT1 Gene Expression in Patients of Acquired Aplastic Anemia with Different TCM Syndromes and Correlation Study
下载PDF
导出
摘要 目的观察端粒保护蛋白1(POT1)在不同证型获得性再生障碍性贫血(再障)患者外周血单个核细胞的表达水平,探讨其与获得性再障及其中医辨证分型之间的关系。方法采用实时荧光定量聚合酶链反应(RT-qPCR)检测52例不同证型获得性再障患者和20例正常对照组外周血单个核细胞中POT1 mRNA表达情况,并进行相关性分析。结果获得性再障患者POT1 mRNA表达情况较正常对照组明显减低(P<0.05)。肾阴虚型POT1 mRNA表达水平低于肾阳虚型,肾阴阳两虚型最低,并且与年龄有明显相关性(r=0.374,P=0.038)。结论 POT1参与了获得性再障的发病经过,其在外周血单个核细胞的表达水平与获得性再障的中医证型有一定相关性。 Objective To observe the mRNA expression levels of POT1 in peripheral blood mononuclear cells of acquired aplastic anemia patients with different syndrome,and explore its relationship with acquired aplastic anemia and its TCM syndrome.Methods Peripheral blood mononuclear cells of 52 cases with acquired aplastic anemia and 20 cases as control group were collected to detect mRNA expression of POT1 by using real-time quantitative polymerase chain reaction(RT-qPCR),and its relation with TCM syndrome was analyzed.Results The expression levels of POT1 mRNA in patients with acquired aplastic anemia were lower significantly than control group(P〈0.05).The expression levels of POT1 mRNA in patients with deficiency of kidney-yin were lower than patients with deficiency of kidney-yang,and it was lowest in patients with kidney deficiency of both yin and yang.There was significant correlation between the expression levels of POT1 mRNA and age(r =0.374,P =0.038).Conclusion The changes in expression levels of POT1 play a role in the pathogenesis of acquired aplastic anemia.There is correlation between mRNA expression level of POT1 in peripheral blood mononuclear cells and TCM syndrome.
出处 《中国中医药信息杂志》 CAS CSCD 2013年第8期18-20,35,共4页 Chinese Journal of Information on Traditional Chinese Medicine
基金 国家科技支撑计划(2007BAI20B06)
关键词 获得性再生障碍性贫血 中医证型 外周血单个核细胞 端粒保护蛋白1 acquired aplastic anemia TCM syndrome peripheral blood mononuclear cells POT1
  • 相关文献

参考文献13

  • 1Brummendorf TH,Macie Jewski JP,Mak J,et al. Telomere length in leukocyte subpopulations of patients with aplastic anemia[J]. Blood,2001,97(4):895-900.
  • 2Young NS,Bacigalupo A,Marsh JC. Aplastic anemia: pathophysiology and treatment[J]. Biol Blood Marrow Transplant,2010,16(1):S119-125.
  • 3Baumann P,Cech TR. Pot1,the putative telomere end-binding protein in fission yeast and humans[J]. Science,2001,292(5519):1171-1175.
  • 4张之南,沈悌.血液病诊断及疗效标准[M].3版,北京,北京科学出版社,2008:232-235.
  • 5Young NS. Telomere biology and telomere diseases:implications for practice and research[J]. Hematology Am Soc Hematol Educ Program,2010(1):30-35.
  • 6Scheinberg P,Cooper JN,Sloand EM,et al. Association of telomere length of peripheral blood leukocytes with hematopoietic relapse,malignant transformation,and survival in severe aplastic anemia[J]. JAMA,2010,304:1358-1364.
  • 7De Lange T. T-loops and the origin of telomeres[J]. Nat Rev Mol Cell Biol,2004,5(4):323.
  • 8Colgin LM,Baran K,Baumann P,et al. Human POT1 facilitates telomere elongation by telomerase[J]. Curr Biol,2003,13:942- 946.
  • 9Poncet D,Belleville A,de Roodenbeke CT,et al. Changes in the expression of telomere maintenance genes suggest global telomere dysfunction in B-chronic lymphocytic leukemia[J]. Blood,2008,111:2388-2391.
  • 10帖君,房殿春,宁晓燕,罗元辉.人端粒保护蛋白在胃癌中的异常表达及其临床意义[J].胃肠病学和肝病学杂志,2006,15(1):12-14. 被引量:2

二级参考文献38

  • 1罗仁,钟洪.疾病人群的虚证特点[J].辽宁中医杂志,1996,23(4):154-155. 被引量:12
  • 2赵伟康.中药延缓衰老的作用特点与研究途径[A].陈可冀主编.迈向21世纪的中西医结合[C].北京:中国医药科技出版社,1991.298.
  • 3彭铭泉.中国药膳大典[M].成都:四川科学技术出版社,1988.116-130.
  • 4Miura T, Mattson MP, Rao MS. Cellular lifespan and senescence signaling in embryonic stem cells. Aging Cell,2004, 3(6):333-343.
  • 5Maser RS, DePinho RA. Connecting chromosomes, crisis, and cancer. Science, 2002, 297(5581): 565-569.
  • 6Smogorzewska A, de-Lange T. Regulation of telomerase by telomeric proteins. Annu Rev Biochem, 2004, 73: 177-208.
  • 7Baumann P, Podell E, Cech TR. Human Pot1 (protection of telomeres) protein: cytolocalization, gene structure, and alternative splicing. Mol Cell Biol, 2002, 22(22): 8079-8087.
  • 8Baumann P, Cech TR. Pot1, the putative telomere end-binding protein in fission yeast and humans. Science, 2001, 292(5519): 1171-1175.
  • 9Loayza D, De-Lange T. POT1 as a terminal transducer of TRF1 telomere length control. Nature, 2003, 423(6943): 1013-1018.
  • 10Liu D, Safari A, O′Connor MS, et al. PTOP interacts with POT1 and regulates its localization to telomeres. Nat Cell Biol, 2004, 6(7): 673-680.

共引文献17

同被引文献9

引证文献2

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部