摘要
目的检测基质金属蛋白酶-2(MMP-2)与基质金属蛋白酶抑制剂-2(TIMP-2)在宫颈鳞状细胞癌组织中的表达,探讨宫颈鳞状细胞癌发展与临床分期及淋巴结转移中的意义。方法应用免疫组化法和图像分析方法检测MMP-2与TIMP-2在正常宫颈、宫颈上皮内瘤变(CIN)及宫颈鳞癌组织中的表达情况,并对临床病理特征进行对比分析。结果 MMP-2在正常宫颈、CIN、宫颈鳞癌组织中表达阳性率逐渐增高(P<0.05),MMP-2在宫颈鳞癌中的表达与分化程度、临床分期及淋巴结转移正相关(P<0.05);TIMP-2在宫颈鳞癌中的表达与临床分期及淋巴结转移负相关(P<0.05);MMP-2与TIMP-2在宫颈鳞癌中的表达呈负相关关系(P<0.05)。结论 MMP-2及TIMP-2与宫颈鳞癌的发生、发展相关,联合检测MMP-2及TIMP-2对预测CIN的转归及判断宫颈鳞癌的预后可提供一定的理论依据,并为宫颈癌的基因治疗提供靶点。
Objective To discuss the expression and clinical significance of MMP-2 and TIMP-2 in cervical squa- mous cell carcinoma. Methods Expression of MMP-2 and TIMP-2 are detected in normal cervix tissue,CIN and cervi- cal squamous cell carcinoma by immunohistochemical method. Results Expression of MMP-2 in normal cervical,CIN, cervical squamous cell carcinoma was gradually increased （P〈0.05）, expression of MMP-2 in cervical squamous cell carcinoma and the degree of differentiation,clinical stage and lymph node metastasis showed positive correlation （P〈 0.05） ;expression of TIMP-2 in cervical squamous cell carcinoma of clinical stage and lymph node metastasis showed negative correlafion （P〈0.05） ;expression of MMP-2 and TIMP-2 in cervical squamous cell carcinoma showed negative correlation （P〈0.05）. Conclusion Occurrence and development of cervical squamous cell carcinoma may be associat- ed with MMP-2 and TIMP-2. Detection of MMP-2 and TIMP-2 to predict the outcome of CIN and to determine the prognosis of cervical squamous cell carcinoma can provide a theoretical basis.
出处
《中国实验诊断学》
2013年第7期1254-1257,共4页
Chinese Journal of Laboratory Diagnosis
关键词
基质金属蛋白酶-2
基质金属蛋白酶抑制剂-2
宫颈上皮内瘤变
宫颈鳞癌
Matrixmetallo proteinase-2（MMP-2）
Tissue inhibitor of metallo-proteinase-2（TIMP-2）
Cervical intraepithelial neoplasias（CIN）
Cervical Carcinoma
