摘要
目的建立微孔板阿尔玛蓝显色法检测速分枝杆菌药物敏感性的方法,评价其临床检测效能。方法用GenoType Mycobacterium CM分枝杆菌菌种鉴定系统和测序法筛选20株龟分枝杆菌临床株、16株脓肿分枝杆菌临床株作为实验株,阿尔玛显色法检测龟、脓肿分枝杆菌对阿米卡星、头孢西丁、环丙沙星、克拉霉素、多西环素、亚胺培南、利奈唑胺、磺胺甲恶唑和妥布霉素9种抗生素的最低抑菌浓度,以微量肉汤稀释法为标准评价符合率和MIC结果解释的一致率。结果微孔板阿尔玛蓝显色法检测9种抗生素的MIC值分布与微量肉汤稀释法MIC值符合率分别为100%(阿米卡星、环丙沙星、利奈唑胺)、97.2%(头孢西丁、克拉霉素、多西环素、妥布霉素)、94.4%(亚胺培南)和88.9%(磺胺甲恶唑)(±1log2范围内);MIC结果解释一致率头孢西丁和利奈唑胺为94.4%,妥布霉素和磺胺甲恶唑为97.2%,其余5种药物药敏结果解释均完全一致。结论微孔板阿尔玛蓝显色法是一种简便、易于判读、准确的MIC测定方法,可用于速生长分枝杆菌临床分离株的快速药敏测试。
Objective To evaluate the efficacy of micoplate Alamar blue assay （MABA） for antimicrobial susceptibility testing of rapidly growing mycobacteri strains in shenzhen. Methods 36 rapidly growing mycobacteria strains isolated from clinical specimens were collected. Species identification was carried by GenoType Mycobacterium CM of Hain and sequencing. Minimum inhibitory concentrations （MIC） of amikacin, cefoxitin, ciprofloxacin, clar- ithromycin, doxycycline, imipenem, linezolid, sulfamethoxazole and tobramycin were determined by MABA, and the results were compared with the reference broth microdilution method according to CLSI M24 - A guidelines. Results Agreement between broth microdilution and MABA MICs within ~ 1 log2 dilution for nine drugs were respectively 100% for amikacin, ciprefloxacin and linezolid, 97.2% for cefoxitin, clarithromycin, doxycycline and tobramycin, 94.4% for imipenem and 88.9% for sulfamethoxazole. The agreement by interpretative category among CLSI and MABA broth microdilution methods were respectively 94. 4% for cefoxitin and linezolid, 97.2% for sulfamethoxazole and tobramyein, 100% for the others 5 antibiotics. Conclusion MABA provide an accurate and easy - to - read MIC determination method and can be used for rapid detection of the antibiotic susceptibility testing for rapidly growing my- cobacteria.
出处
《现代医院》
2013年第7期71-74,共4页
Modern Hospitals
基金
深圳市科技计划项目(编号:JCYJ20130402154801094)
