摘要
目的 :探讨食管癌组织中多药耐药相关蛋白基因 (MRP)表达与其病理特征的关系。方法 :应用逆转录 多聚酶链反应 (RT PCR)方法 ,对 5 1例食管癌、癌旁及 3 3枚淋巴结组织中MRP基因的表达进行检测分析。结果 :食管癌组织中MRP阳性率 ( 3 5 3 %)高于癌旁组织 ( 3 9%)。MRP表达阳性与肿瘤大小、病理类型及淋巴转移与否无明显关系 ,但TNMⅢ、Ⅳ期和肿瘤侵犯深肌层以上的MRP阳性率增高 ;低分化食管癌的MRP阳性分布明显高于高分化肿瘤 (P <0 0 5 )。来自 13例患者的 2 1枚转移淋巴结中 ,5例癌组织MRP表达阳性的 6枚转移淋巴结组织MRP均阳性 ,12枚非转移淋巴结中未见MRP阳性表达 ;对部分病例随访发现 ,MRP阳性者肿瘤复发、转移的发生率 ( 4 2 9%)高于MRP阴性者 ( 19%)。结论 :食管癌组织中存在着原发性MRP基因表达 ,转移淋巴结组织的MRP基因表达与原发灶具有一致性 ,MRP的阳性表达除了表现肿瘤的多药耐药外 ,还可能是食管癌恶性行为的观察指标 ,提示预后不良。
Objective: To investigate the expression of multidrug resistance associated protein MRP gene and its relation to pathological features of esophageal carcinoma Methods: The samples of tumor tissue, paracancerous tissue and 33 lymph node from 51 patients with esophageal carcinoma were analyzed for MRP gene expression by RT PCR Results: The MRP positive rate of the tumor tissue was significantly different from that of the paracancerous tissue (P< 0 05) The expression of MRP gene was not associated with tumor size, pathomorphology and lymph node metastasis The MRP positive rate was higher in patients with tumor of stages Ⅲand Ⅳand deep musclaris or fibro membrane invaded by tumor than in those with tumor at stages I and II and shallow musclaris invasion The positive expression of MRP was markedly higher in undifferentiated tumor than in the well differentiated one (P< 0 05) The MRP positive rate of metastatic lymph nodes in MRP positive tumor tissues was 100% There was no positive MRP expression in non metastatic lymph nodes The follow up showed that the relapse or metastatic rate in MRP positive cases was higher than that in MRP negative ones Conclusion: Overexpression of MRP gene in untreated esophageal carcinoma is in accordance with its metastasis to lymph nodes It can be regarded as a prognostic marker of esophageal carcinoma
出处
《第三军医大学学报》
CSCD
北大核心
2000年第3期262-264,共3页
Journal of Third Military Medical University
关键词
食管癌
MRP
基因表达
病理特征
esophageal carcinoma
MRP gene expression
pathological feature
