摘要
目的:检测鼻咽癌患者外周血中Treg细胞与Th17细胞的比例以及相关细胞因子如IL-6、TGF-β1的变化。方法:选取我院鼻咽癌患者50例,另选取健康志愿者50例,鼻咽炎患者50例作为对照。采用流式细胞术检测外周血中Th17细胞和Treg细胞百分率,ELISA法检测血清中细胞因子TGF-β1、IL-6的水平,分析其相关性。结果:鼻咽癌外周血中Treg和Th17细胞占CD4+T细胞的比例均升高,与鼻咽炎组、正常对照组比较,均有显著性差异(P<0.05);且Treg细胞与Th17细胞呈正相关(P<0.05);鼻咽癌外周血中TGF-β1、IL-6的水平均明显高于鼻咽炎组、正常对照组,均有显著性差异(P<0.05);鼻咽癌患者外周血Th17细胞、Treg细胞分别与血清中IL-6、TGF-β1水平呈正相关(P<0.05)。结论:鼻咽癌患者外周血中Treg、Th17细胞比例升高,且两者呈正相关;鼻咽癌患者患者血清中TGF-β1、IL-6水平升高,可能影响Treg与Th17细胞的平衡。
Objective：To investigate the proportion of Treg cells and Th17cells in the peripheral blood of patients with nasopharyngeal carcinoma and to detect changes of related cytokines such as IL-6 and TGF-beta1.Methods：Fifty patients with nasopharyngeal carcinoma in our hospital were selected and 50cases of nasopharyngitis and 50healthy volunteers were also enrolled as control groups.Percentage of Th17cells and Treg cells in the peripheral blood were detected by flow cytometry,serum cytokine TGFβ1,IL-6levels were detected with Percentage ELISA assay.Results：For nasopharyngeal carcinoma cases,percentage of Treg and Th17cells in peripheral blood CD4＋T cells were increased,showing significant difference compared with the nasopharyngitis groups and normal control group（P0.05）.Besides,in peripheral blood of nasopharyngeal carcinoma patients,Treg cells and Th17cells were positively correlated with each other（P0.05）.Conclusions：In peripheral blood of patients with nasopharyngeal carcinoma,proportion of Treg cells and Th17cells were both increased,and positively correlated with each other,for this patients,the increased TGF-β1,IL-6levels may affect the balance of Treg and Th17 cells.
出处
《海南医学院学报》
CAS
2013年第9期1194-1196,共3页
Journal of Hainan Medical University
基金
琼卫2012PT-29~~
