摘要
致病疫霉(Phytophthora infestans)引起的晚疫病是马铃薯的一种毁灭性病害。有效控制马铃薯晚疫病需要明确致病疫霉的群体遗传结构特征。采用8对SSR引物对采自福建省福州、长乐、漳州2010年分离的95株马铃薯致病疫霉进行遗传多样性分析。结果共检测出21个等位基因和26个基因型。三个地点致病疫霉菌群体间的平均遗传分化系数FST为0.22,在8个位点中有5个位点的等位基因频率分布差异显著。三个群体的观测纯合度小于期望纯合度,观测杂合度大于期望杂合度,以无性生殖为主。结果表明福建群体的遗传多样性高,群体间的存在较高的遗传分化度。
Late blight, caused by P. infestans, is the most distructive disease of potato （Solanum tuberosum）. Effec- tive management of potato late blight is aided by an understanding of the characteristics of the contemporary pathogen population. 95 isolates were sampled from three locations in Fujian province in 2010 and we assayed the isolates with eight simple-sequence repeat （SSR） markers. Twenty-one alleles and 26 genotypes were detected among the 95 iso- lates. FST measurement of population differentiation was 0.22 and there were significant differences in allelic frequency in five out of eight loci. In all these populations, an excess of heterozygosity and deficiency of homozygosity was found, suggesting clonal reproduction of the pathogen. Results also suggest a high genetic variation in the Fujian population of P. infestans and moderate to high genetic differentiation among the subpopulations from different locations.
出处
《激光生物学报》
CAS
CSCD
2013年第3期267-272,共6页
Acta Laser Biology Sinica
基金
国家现代农业马铃薯产业技术体系(编号:CARS-10)
国家自然科学基金(编号:31071655)
福建省教育厅重点项目(编号:JA12093)
关键词
致病疫霉
SSR基因型
遗传多样性
Phytophthora infestans
SSR genotyping
genetic diversity
