摘要
目的:研究槐米中槲皮素对焦虑模型小鼠的保护作用。方法:以不确定刺激复制小鼠焦虑模型。小鼠跳台实验分为空白对照(等容生理盐水)组、模型(等容生理盐水)组、地西泮(10mg/kg)组与槲皮素高、低剂量(60、15mg/kg)组,灌胃给药,每天1次,在给药第6天进行跳台训练实验,第7天为正式测试实验。小鼠明暗箱实验分为空白对照(等容生理盐水)组、模型(等容生理盐水)组、地西泮(10mg/kg)组与槲皮素高、低剂量(60、15mg/kg)组,灌胃给药,每天1次,末次给药后进行明暗箱实验。以高效液相-荧光检测法测定小鼠脑内γ-氨基丁酸(GABA)、谷氨酸(Glu)、5-羟色胺(5-HT)的含量。结果:与模型组比较,槲皮素高剂量组小鼠穿箱次数显著增加(P<0.05);槲皮素高、低剂量组小鼠测试期错误反应次数显著减少(P<0.01),小鼠脑内GABA、Glu含量显著增加(P<0.01)。结论:槲皮素具有明显的抗焦虑作用,其机制与提高小鼠脑内GABA、GLu含量有关。
OBJECTIVE： To study the protective effects of quercetin of Sophora japonica on anxiety model mice. METHODS： Anxiety model was induced by uncertain stimulation. In step-down test ,mice were divided into blank control group（equal volume of normal saline） , model group （equal volume of normal saline）, diazeparn group （10 mg/kg） and quercetin high-dose and low-dose groups （60, 15 mg/kg）. They were given relevant medicines intragastrically once a day. Model mice were included in step-down training test on sixth day of medication, and on seventh days for formal test. In light-dark box , mice were divided into blank control group（equal volume of normal saline）, model group （equal volume of normal saline）, diazepam group（10 mg/kg） and quercetin high-dose and low-dose groups（60, 15 mg/kg）. They were given relevant medicines intragastrically once a day. The test was conducted after last administration. The contents of y-propalanine（GABA）, glutamate （Glu） and 5-serotonin（5-HT） were de- termined by HPLC-fluorescence assay. RESULTS： Compared with model group, the number of light-black transitions increased sig- nificantly in quercetin high-dose group（P〈0.05） ;the number of error response decreased significantly in quercetin high-dose and low-dose groups （P〈0.01）, while the contents of GABA and Glu in cerebral tissue of mice increased significantly（P〈0,01）.CON- CLUSIONS： Quercetin has obvious anti-anxiety effects, and the mechanism may be associated with the increase of GABA and Glu contents in cerebral tissue of mice.
出处
《中国药房》
CAS
CSCD
2013年第31期2905-2907,共3页
China Pharmacy
