摘要
目的建立HPLC法同时测定心脑康胶囊(丹参、赤芍、葛根、川芎、红花等)中丹参素、芍药苷、葛根素、羟基红花黄色素A、川芎嗪和阿魏酸的方法。方法 Agilent HC-C18色谱柱(4.6 mm×250 mm,5μm),流动相为0.2%甲酸水(A)-甲醇(B),梯度洗脱(0～12 min,18%～35%B;12～22 min,35%～45%B;22～45 min,45%B);柱温30℃;进样量20μL,体积流量0.6 mL/min;芍药苷和葛根素检测波长为254 nm,丹参素和川芎嗪为280 nm,阿魏酸为320 nm,羟基红花黄色素A为403 nm。结果芍药苷、葛根素、羟基红花黄色素A在2.5～200μg/mL(r2=0.999 8),阿魏酸在2.91～233μg/mL(r2=0.999 8),丹参素在1.66～133μg/mL(r2=0.999 9)、川芎嗪在0.81～130μg/mL(r2=0.999 8)质量浓度范围内与峰面积具有良好的线性关系,平均回收率均大于97%。结论该方法准确可靠,可行性及重复性良好,可作为心脑康胶囊的质量控制方法。
AIM To establish an HPLC method for simultaneously determining danshensu,peoniflorin,puerarin,hydroxysafflor yellow A,tetramethylpyrazine and ferulic acid in Xinnaokang Capsules（Salviae miltiorrhizae Radix et Rhizoma,Paeoniae Radix rubra,Puerariae lobatae Radix,Chuanxiong Rhizoma,Carthami Flos,etc.）.METHODS The analysis was performed on Agilent HC-C18 column（4.6 mm×250 mm,5 μm）,and the mobile phase consisted of methanol-0.2% formic acid.The flow rate was 0.6 mL/min and detection wavelengths were set at 254 nm for peoniflorin and puerarin,280 nm for danshensu and tetramethylpyrazine,320 nm for ferulic acid and 403 nm for hydroxysafflor yellow A.RESULTS The samples showed a good relationship in the ranges of 2.5-200 μg/mL for peoniflorin,puerarin and hydroxysafflor yellow A,2.91-233 μg/mL for ferulic acid,0.81-130 μg/mL for tetramethylpyrazine and 1.66-133 μg /mL for danshensu.All of the average recoveries were more than 97%.CONCLUSION The method is accurate,reliable and reproducible and can be used for quality control of Xinnaokang Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2013年第8期1672-1675,共4页
Chinese Traditional Patent Medicine
基金
国家科技支撑计划课题(2008BAI51B01)
深圳市科技工贸和信息化委员会项目(CXB201005260069)
西北大学大学生创新基金(2011007)
关键词
心脑康胶囊
丹参素
芍药苷
葛根素
川芎嗪
阿魏酸
羟基红花黄色素A
Xinnaokang Capsules
danshensu
peoniflorin
puerarin
tetramethylpyrazine
ferulic acid
hydroxysafflor yellow A
