摘要
目的 检测恶性疟原虫裂殖子表面蛋白 1基因在减毒鼠伤寒直菌X40 6 4株中的表达。方法 将 7个克隆有恶性疟原虫裂殖子表面蛋白 1基因片段的表达质粒 pQEM用氯化钙法转化到修饰系统正常、限制系统缺陷的鼠伤寒杆菌LB5 0 0 0中 ,再通过专一性噬菌体P2 2 转导入腺苷酸环化酶基因、环腺苷酸受体基因双重突变的减毒鼠伤寒杆菌X40 6 4株中 ,用质粒酶切、Westernblot鉴定构建的重组菌株、测定重组菌株的表达。结果 成功地构建了 7个重组减毒鼠伤寒杆菌X40 6 4(pQEM )株 ,重组pQEM质粒在减毒鼠伤寒杆菌X40 6 4株中获得了表达。结论 X40 6 4(pQEM )
Objective To examine the expression of merozoite surface protein 1(MSP 1) gene of Plasmodium falciparum merozoite in attenuated Salmonella typhimurium X4064 strain. Methods Seven pQEM plasmids cloned with MSP 1 gene fragments were firstly transformed into S. typhimurium LB5000, a restriction negative, modification positive strain, to be modified and then transduced into △cya△crp double mutant S. typhimurium X4064 by using bacteriophage P 22 . Restriction enzyme analysis and Western blot were used to examine the construction and expression of these recombinant strains.Results Seven recombinant strains of S. typhimurium X4064(pQEM) were successfully constructed. The MSP 1 protein fragments were expressed in these recombinant strains.Conclusion The recombinant S. typhimurium X4064(pQEM) strains could be considered as candidates for malaria vaccine.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2000年第3期186-189,共4页
Chinese Journal of Infectious Diseases
基金
解放军总后勤部卫生部高新技术基金
关键词
恶性疟原虫
裂殖子表面蛋白-1
减毒鼠伤寒杆菌
Plasmodium falciparum
Merozoite surface protein 1
Attenuated Salmonella typhimurium
Construction
