摘要
目的研究普罗布考对阿霉素大鼠足细胞相关分子nephrin及podocin表达的影响。方法将36只SD大鼠分为正常对照组(12只)、肾病模型组(12只)和普罗布考干预模型组(12只),采用鼠尾静脉注射阿霉素的方法建立阿霉素大鼠肾病模型。4周末检测各组大鼠24 h尿蛋白定量,光镜下观察肾脏病理学改变,Western blot技术检测nephrin及podocin的表达。结果肾病模型组和普罗布考干预组24 h尿蛋白定量均高于正常对照组(P<0.05),但普罗布考干预组24 h尿蛋白定量低于肾病模型组(P<0.05)。肾病模型组和普罗布考干预组肾小球nephrin、podocin的表达均低于正常对照组(P<0.05),且普罗布考干预组高于肾病模型组(P<0.05)。结论普罗布考可能通过减轻足细胞损伤实现对阿霉素肾病大鼠肾脏的保护作用。
Objective To study the effects of probucol on podocin and nephrin expression in rats with adriamycin-induced nephrosis.Methods A total of 36 SD rats were randomly divided into three groups：control group（CG group,n =12）,nephrosis group（NS group,n =12）and probucol group（NSP group,n =12）.The nephrosis models were established by caudal vein injection of adriamycin.Twenty-four h urine protein quantification was performed at the end of the forth week.Light microscope was employed to investigate the pathological changes of the glomerulus,Western blot was carried out to determine the protein expression of podocin and nephrin.Results Twenty-four h urine protein was significantly increased in NS group and NSP group compared with the CG rats（P 0.05）,and less urinary protein was detected in NSP group than in NS group（P 0.05）.Protein expression of nephrin and podocin was significantly decreased in NS and NSP rats than in CG rats（P 0.05）,while protein expression of these two components was up-regulated in NSP rats compared with the NS rats（P 0.05）.Conclusion Probucol may achieve the protective effect on adriamycin-induced nephrosis by relieving the podocyte injury.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2013年第8期693-696,共4页
Journal of China Medical University
基金
辽宁省教育厅高校科研计划(L2010581)
