摘要
目的 :建立了一种反相离子对色谱法测定诺氟沙星滴眼液中的诺氟沙星和有关物质。方法 :用SpherisorbC18ODS柱 ,甲醇 0 .0 5mol·L-1磷酸盐缓冲液 0 .0 5mol·L-1四丁基溴化铵 (2 5∶75∶4,pH =2 .75 )为流动相 ,紫外检测波长为 2 80nm。结果 :诺氟沙星线性范围 :0 .12～ 1.0 0 μg ,r =0 .9994,平均回收率为 98.0 % ,RSD为 1.0 % (n =5 )。结论 :该法准确、快速、简便 ,可用于诺氟沙星滴眼液中的诺氟沙星的含量测定。
OBJECTIVE:An ion pair reversed phase HPLC in the seperation and determination of norfloxacin and relative substances in eye drops were developed.METHODS:Spherisorb C 18 ODS as stationary phase and methanol 0.05 mol·L -1 KH 2PO 4(pH was adjusted to 2.75 with phosphoric acid) 0.05 mol·L -1 tetrabutyammonium bromide(25∶75∶4) as the mobile phase were adopted and the detection wavelenth was 280 nm.RESULTS:The linear range of nofloxacin was 0.12 ～ 1.00 μg( r = 0.999 4 , n =5).The average recovery was 98.0% , RSD = 1.0% ( n =5).CONCLUSIONS:The method is accurate,rapid and simple for the determination of nofloxacin in eye drops.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2000年第7期402-404,共3页
Chinese Journal of Hospital Pharmacy
