摘要
在猪输卵管上皮细胞(pOECs)与猪卵巢皮质细胞(pOCCs)共培养体系中进行猪卵子体外成熟(IVM),研究比较了pOECs与pOCCs对猪卵母细胞体外成熟(IVM)、体外受精与胚胎发育的影响。结果显示:pOCCs或pOECs参与猪卵子体外成熟可显著提高卵丘扩散与MⅡ期卵子的比例(P<0.05),然而GV期卵子的比例却没有显著减少(P>0.05);pOCCs组内成熟卵子的GSH含量显著高于其余各组(P<0.05),而GSH在pOECs组与对照组之间差异不显著(P>0.05);尽管pOCCs和pOECs共成熟可显著降低多精入卵的比例(P<0.05),但是受精率与雄原核形成率(MPN)在各组间却无显著差异(P>0.05);pOCCs和pOECs共培养均可以显著提高卵裂率与囊胚发育率(P>0.05),然而囊胚细胞数在处理组与对照组间没有显著性差异(P>0.05)。研究认为,pOCCs与pOECs共培养体系能显著提高猪卵母细胞IVM质量,降低多精入卵的发生,并提高囊胚发育率。
Porcine oocytes were co-cultured respectively with porcine ovarian cortex cells（pOCCs） and porcine oviductal epithelial cells（pOECs） during in vitro maturation（IVM）.The effects of pOCCs and pOECs co-culture during IVM on the maturation,fertilization and embryos development in vitro were studied.The results showed that cumulus significantly expanded and matured and MⅡoocytes in the co-culture groups were higher than that in the control group（P＆lt;0.05）,whereas there was no significant difference in unmatured GV oocytes between the groups（P＆gt;0.05）.In addition,the GSH concentration in the pOCCs group was higher than that in other groups（P＆lt;0.05）.After IVF,polyspermy in the co-culture groups were lower than that in the control group（P＆lt;0.05）,and there were no significant difference in both fertility and male pronucleus（MPN） formation between the groups（P＆gt;0.05）.In conclusion,the presence of the pOCCs and pOECs during IVM enhanced the maturation quality of the porcine oocytes,reduced the polyspermy,and increased the percentages of blastocyst.
出处
《浙江农业学报》
CSCD
北大核心
2013年第4期705-709,共5页
Acta Agriculturae Zhejiangensis
基金
浙江省公益性项目(2010C32068)
国家自然科学基金(31172210)
关键词
输卵管上皮细胞
卵巢皮质细胞
体外成熟
卵母细胞
猪
porcine oviductal epithelial cells（pOECs）
porcine ovarian cortex cells（pOCCs）
in vitro maturation（IVM）
oocyte
porcine
