摘要
牡丹（Paeonia suffruticosa Andr.）为芍药科（Paeoniaceae）牡丹组（Sect.Moutan）落叶亚灌木,是原产我国的名贵木本观赏花卉,素有＂国色天香＂、＂花中之王＂的美称,是中国的候选国花[1]。牡丹广泛的用途使其需求量日益增加,但牡丹很多品种不结实或结实率极低,目前主要以分株繁殖和嫁接繁殖为主,存在繁殖速度慢、苗木质量参差不齐等问题,严重制约着牡丹的规模化生产,牡丹的良种扩繁己成为亟待解决的问题。
The tissue-cultured seedlings of tree peony‘Wulong Pengsheng’were used to study the effects of different plant growth regulators,culture methods,and holdfast on rooting.The morphological structure change during rooting was also observed using the method of paraffin section.The result showed that the best combination of plant growth regulators for rooting was IBA 3.0 mg.L-1 ＋ NAA 0.6 mg.L-1.The treatment under the temperature of 4℃ for ten days was benefit to rooting,and the rate could reach 75.67%.It was identified that the adventitious root primordia of shoot in vitro originated from the vascular cambium cells,especially,the cross areas of cambium and pith ray and they started to differentiate at the 5th day and lasted to the 12th day.If the shoots were cultured in the root inducing medium for 12 days,it would lead to not only descend of rooting rate,but also showing callus of stem base,and leaf senescent.However,if they were transferred into the medium without hormone in time,the root primordial protruded the epidermis and developed normally after 5 days’culture.
出处
《林业科学研究》
CSCD
北大核心
2013年第4期516-520,共5页
Forest Research
基金
河南省重点科技攻关项目"牡丹组培工厂化育苗关键技术研究"(112102110021)
关键词
牡丹
'乌龙捧盛’
组培苗
生根
解剖学
Paeonia suffruticosa
Paeonia suffruticosa ‘WuLong PengSheng’
regenerated shoots
rooting
anatomy
