摘要
目的 观察及评估血小板源生长因子 (platelet derivedgrowthfactor,PDGF)和转化生长因子 β1(transforminggrowthfactor,TGF β1)及其相应受体在视网膜表面膜的分布特征及临床意义。方法对 9例孔源性视网膜脱离患者行玻璃体切除术同时获得 2 5块视网膜表面膜 ,按病程分为早期膜(<2个月 )、中期膜 (2～ 6个月 )和晚期膜 (>6个月 ) ;采用 8种抗体 ,即PDGF、TGF β1、PDGFα和 β受体、TGF βⅠ和Ⅱ型受体、Ⅰ和Ⅲ型胶原 ,按研究目的配对后进行胶体金双重标记。 结果 PDGF和TGF β1标记程度在早、晚期膜强 ,中期膜总体上呈现弱 ;增生性玻璃体视网膜病变 (proliferativevitreoretinopathy,PVR)C2 ～C3 膜标本的 2个生长因子阳性标记程度较PVRD1～D3 膜标本强 ;PDGF和TGF β1与Ⅰ和Ⅲ型胶原的胶体金颗粒常聚在同一部位 ,且标记程度一致。 4个生长因子受体阳性标记的细胞是一类胞浆丰富、带或不带色素颗粒的上皮样细胞 ,且细胞周围多伴有生长因子的阳性标记。结论 PDGF和TGF β1以浓度的变化自始至终参与膜的发生、发展 ;上皮样细胞是一种功能活跃细胞 。
Objective To investigate the ultrastructural distribution of platelet derived growth factor (PDGF), transforming growth factor β 1 (TGF β 1) and their receptors in epiretinal membranes (ERM) and discuss the clinical significance of the distribution Methods 25 membrane specimens were surgically removed by vitrectomy from 9 patients with rhegmatogenous retinal detachment Double staining techniques of immunoelectromicroscopy for eight antibodies (PDGF, TGF β 1, PDGF receptor subunits α and β, TGF β subunits Ⅰ and Ⅱ, type Ⅰ and Ⅲ collagen) were used in these specimens as previously designed Results PDGF and TGF β 1 staining appeared to be stronger on early (<2 months) and late (>6 months) stage membranes than that of middle (2～6 months) stage of membranes, and the immunostaining intensity inverted with the degree of proliferative vitreoretinopathy (PVR) We found that the gold particles of PDGF and TGF β 1 tended to get together with that of type Ⅰ and Ⅲ collagen In addition, the stainings of four growth factor receptors were frequently positive in a type of epithelial like cells with rich cytoplasm components, and also the gold particles of PDGF and TGF β 1 were found around these cells Conclusions A concentration change of PDGF and TGF β 1 exists in development of ERM throughout The epithelial like cells are of a type of active cells, and autocrine and paracrine activity may be involved in ERM pathogenesis PDGF and TGF β 1 influence the contractile activity of ERM
出处
《中华眼科杂志》
CSCD
北大核心
2000年第5期369-371,I024,共3页
Chinese Journal of Ophthalmology
