摘要
目的采用实时荧光PCR检测耐甲氧西林金葡菌(MRSA)杀白细胞素(PVL)基因,并分析其对抗菌药物敏感性,指导临床合理用药。方法采用双重实时荧光PCR技术对血液和脓液分离的40株MRSA进行PVL基因检测,并对PVL基因阳性菌株感染患者的临床资料进行分析;VITEK2-compact全自动微生物测试仪进行药敏试验。结果 40株MRSA检出7株PVL基因阳性,阳性率17.5%,PVL基因阳性菌株所致的感染分别为坏死性皮肤软组织感染3例,血流感染3例和乳腺脓肿1例。MRSA携带或不携带PVL基因菌株的药敏试验结果有所不同,未检出对万古霉素不敏感菌株。结论 MRSA携带或不携带PVL基因对抗菌药物敏感性有差异,本地区MRSA-PVL基因阳性率较高,应加强监测。
Objective We developed a real-time PCR assay for detection of panton-valentine leukocidin（PVL）gene of methicillin-resistant Staphylococcus aureus.The antimicrobial susceptibility was also tested for these pathogens for reasonable clinical prescription.Methods A total of 40 MRSA strains were isolated from blood and pus.The MRSA strains carrying Panton-Valentine leukocidin gene（PVL-MRSA）were identified by duplex real-time PCR.The patients carrying PVL-MRSA were surveyed.Antimicrobial susceptibility testing was tested with VITEK2-Compact system.Results A total of 7（17.5%）PVLMRSA strains were identified from the 40 MRSA stains.These PVL-positive isolates were responsible for necrotizing skin and soft tissue infection（3 cases）,blood stream infection（3 cases）and breast abscess（1 case）.The MRSA strains carrying or not carrying Panton-Valentine leukocidin gene showed different antibiotic resistant patterns.No vancomycin resistant strain was found.Conclusions MRSA strains have different antibiotic resistant patterns dependent on whether they carry Panton-Valentine leukocidin gene or not.The MRSA strains carrying PVL gene are relatively prevalent in this hospital.
出处
《中国感染与化疗杂志》
CAS
北大核心
2013年第4期289-292,共4页
Chinese Journal of Infection and Chemotherapy
基金
广东省深圳市科技计划项目(201203318)
深圳市龙岗区科技计划医疗卫生资助项目(ys2012198)
关键词
耐甲氧西林金黄色葡萄球菌
社区获得性MRSA
杀白细胞毒素
耐药性
实时荧光PCR
methicillin-resistant Staphylococcus aureus
community-associated methicillin-resistant S.aureus
Panton-valentine leukocidin
drug resistance
real-time fluorescent PCR
