摘要
目的检测急性白血病患儿白血病干细胞(LSC)的表面分子CD96的表达,并分析其临床意义。方法分离骨髓中单个核细胞,在此基础上采用分选型流式细胞仪分离CD34+CD38-CD123+的LSC进行培养,采用检测型流式细胞仪检测69例初治急性白血病患儿LSC表面分子CD96的表达,利用R显带技术检测69例患儿染色体核型,收集患儿血常规,检测免疫相关指标等资料。结果 CD96主要在急性髓系白血病(AML)中表达,在急性淋巴细胞白血病(ALL)中也有少量表达,二者之间的表达差异有统计学意义(P<0.05)。维吾尔族患儿CD96的中位表达含量23.4%,汉族患儿CD96的中位表达含量21.2%,表达量在两民族之间差异无统计学意义(P>0.05)。CD96+患儿多伴有预后不良的染色体核型。CD96+患儿初次化疗完全缓解率低于CD96-患儿,而化疗后感染率及复发率明显高于CD96-患儿,差异有统计学意义(P<0.05)。结论急性白血病LSC表面CD96表达阳性的患儿预后欠佳,CD96分子可能是判断急性白血病预后的一个新型指标。
Objective To detect the expression of surface molecule CD96 on stem cell （LSC） in children with acute leukemia, and to explore its clinical significance. Methods Bone marrow mononuclear cells were isolated in 69 children with newly diagnosed acute leukemia. CD34＋CD38CD123＋ LSCs were separated from these cells by flow cytometry （FCM） and then cultured, and CD96 expression on LSCs was detected by FCM. R-banding technique was used to analyze the karyotypes of the 69 children, and the data of their routine blood and immunological tests were collected. Results CD96 was mainly expressed in children with acute myelogenous leukemia, and expressed to a lesser extent in those with acute lymphoblastic leukemia （P〈0.05）. The median expression level of CD96 in Uyghnr children was 23.4%, versus 21.2% in Han children （P〉0.05）. The majority of children with CD96-positive children presented poor-prognosis karyotypes. Compared with CD96-negative children, children with CD96-positive children had a significantly lower complete remission rate （P〈0.05） and significantly higher infection and relapse rates after chemotherapy （P〈0.05）. Conclusions Children with acute leukemia who have CD96-positive LSCs have a poor prognosis. CD96 may be a new indicator of prognosis in children with acute leukemia.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2013年第8期633-637,共5页
Chinese Journal of Contemporary Pediatrics
基金
新疆医科大学研究生创新基金项目(基金编号:MC2010-5)
