摘要
以‘海沃德’猕猴桃组培苗叶片为外植体,建立叶片直接再生体系。结果表明,叶片再生不定芽最佳培养基为MS+3.0mg·L-16-BA+0.1mg·L-1NAA,出芽率达80.95%;不定芽增殖最佳培养基为MS+3.0mg·L-16-BA+0.2mg·L-1NAA+0.1mg·L-1GA3,1～3代平均增殖系数达6.38;最适不定芽生根培养基为1/2MS+0.7mg·L-1IBA,生根率为100%;试管苗移栽成活率为92.38%。试验结果为采用叶盘法进行‘海沃德’猕猴桃的遗传转化奠定了良好基础。
Leaves excised from ＇Hayward＇ kiwifruit were used as explants to establish regeneration system. The results indicated that the maximum adventitious buds regeneration frequency achieved 80.95% on the mediu of MS＋3.0 mg.L-1 6-BA＋0.2 mg.L-1NAA; The most suitable medium for shoot multiplication was MS＋3.0 mg＇L-1 6-BA＋0.2 mg.L-1 NAA＋0.1 mg.L-1 GA3 and the three generations average multiplication coefficient was up to 6.38; 1/2MS media with 0.7 mg.L-1 IBA was the most suitable for the rooting of regenerated shoots, and the rooting rate was 100%. The survival rate of hardened regenerated plantlets was as high as 92.38% after transplanting. The results laid a sound foundation for genetic transformation by means of leaf disc method.
出处
《植物生理学报》
CAS
CSCD
北大核心
2013年第8期759-763,共5页
Plant Physiology Journal
基金
国家自然科学基金项目(30871576)
关键词
'海沃德’猕猴桃
叶片
高频再生体系
＇Hayward＇ kiwifruit （Actinidia deliciosa）
leaf
high frequency regeneration
