摘要
目的:筛选结直肠癌(colorectal cancer,CRC)肝转移miRNA对应的特定基因。方法:收集10例CRC患者肿瘤组织标本,其中同时伴肝转移者5例,无远处器官转移5例。应用miRNA芯片方法对两组样本的miRNA表达差异情况进行研究,并通过实时定量RT-PCR对miRNA的芯片表达差异进行验证,再利用软件预测靶基因。结果:总RNA提取的质量分析显示,每份样品总RNA的A260/A280为2.11～2.15。电泳结果显示,每份样品的总RNA均有清晰的28S和18S条带,RNA完好无降解,质量符合miRNA芯片的质量要求。肝转移组较非转移组筛选出6种CRC表达失调的miRNA,其中上调的为miR-224、miR-1236和miR-622,下调的为miR-155、miR-342-5p和miR-363,miR-224的差异信号值最高(>500)。芯片实验显示,hsa-miR-224在肝转移组呈现显著性上调,P=0.038 2;而hsa-miR-155在肝转移组却呈现显著性下调,P=0.043 2。miR-224的表达倍数在肝转移CRC组织中较非转移组表达上调(Fold change=2.47),P=0.016 6。结论:可调控CRC肝转移的特定基因miR-224的筛选,可作为早期诊断治疗的新手段。
OBJECTIVE： To screen the microRNA related miRNA of colorectal cancer （CRC） with liver metastasis. METHODS： The fresh specimens of primary CRC were collected in 10 patients with 5 cases of hepatic metastasis （group 1） and 5 nor hepatic metastasis （group 2） during operation. The miRNA expression levels between two groups were com- pared by miRNA microarray analysis and further validated using quantitative real-time PCR analysis. RESULTS： A total of six dysregulated miRNAs were identified in liver metastasis CRC comparing with non-metastasis,including 3 up-regula- ted miRNAs （miR-224, miR=1236 and miR-622） and 3 down-regulated miRNAs （miR-155, miR-342-5p and miR-363）. The value of the divergence signal of miR-224 was the highest （〉500）. Hsa-miR-224 was significantly up-regulated（P= 0. 038 2）. The regulation folds of RNA224 was higher in group 1 than that in group 2 （fold change= 2. 47,P=0. 016 6）. CONCLUSION： The screening of miR-224 as a specific gene of CRC with liver metastasis may be used as a new meth- od for early diagnosis and treatment.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2013年第15期1165-1167,1185,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
北京市教委科技计划面上项目(KM2009-10025022)
关键词
微小RNA
基因芯片
结直肠肿瘤
肝转移
特定基因
miRNA
microarray gene chip
colorectal neoplasms
liver metastasis
target gene
