摘要
目的 纯化幽门螺杆菌 (Hp)相对分子质量 (Mr)为 130× 10 3 (CagA)蛋白 ,为单抗及疫苗的研制打下一定基础。方法 用盐酸胍粗提、分子筛层析及凝胶洗脱法纯化Hp970 0 2株Mr 为 130× 10 3(CagA)蛋白并鉴定。用Westernblot方法分析 6 8例患者血清中Mr 为 130× 10 3 (CagA)蛋白的阳性率。结果 纯化蛋白Mr 为 130× 10 3 ,电泳纯 ,并保持良好的抗原性。有 43例患者血清能与纯化的Mr 为 130×10 3 (CagA)蛋白反应 ,其阳性率随着浅表性胃炎、萎缩性胃炎、消化性溃疡和胃癌的排列次序呈逐渐增高趋势。结论 Mr 为 130× 10 3 (CagA)蛋白与胃部疾病的严重性相关。
Objective To purify and identify the CagA (cytotoxin associated gene A) protein of clinical isolate Hp97002 and to investigate the relationships between the purified CagA (molecular weight of 130000) protein and gastric diseases. Methods Using 6M guanidine extract, size exclusion chromatography and elusion from gel was the purified protein (CagA). Sera of 68 patients with gastric diseases (44 with chronic gastritis, 15 with atrophic gastritis, 7 with peptic ulcer disease, 2 with gastric cancer) were obtained, and the serological response to CagA was studied by Western blot using the purified protein. Results The purified protein was molecular weight of 130000 with good antigenicity and revealed basic isoelectric point about 8.1. Among 68 sera, 43 sera could recognize the purified proteins from the patients of chronic gastritis 47.7% (21/44), atrophic gastritis 86.7% (13/15), peptic ulcer disease 100% (7/7), gastric cancer 100% (2/2), the difference was significant (χ 2=13.327, P=0.004). Conclusion CagA (molecular weight of 130000) protein was associated with severe gastric diseases.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2000年第6期496-498,共3页
Chinese Journal of Microbiology and Immunology
基金
浙江省自然科学基金资助项目(397034)
CMB资助项目(96628)
