摘要
目的 了解皮质激素对哮喘患者TH1/TH2功能失衡的调节作用 ,探讨其治疗哮喘的机制。方法 将临床 30例哮喘患儿随机分为甲基强的松龙 (MP)治疗组和对照组 ,用ELISA法测外周血单个核细胞 (PBMC)细胞因子及血清IgE水平 ,用逆转录 聚合酶链反应 (RT PCR)测定细胞因子mRNA表达。结果 MP组临床症状和体征消失时间明显缩短 ;MP对T辅助细胞 (TH)源性细胞因子和血清IgE均有抑制作用 ,但对IL 4、IL 10的抑制作用强于对IFN γ、IL 2的抑制作用 ;MP抑制IL 4mRNA表达程度强于对IFN γ表达抑制。结论 皮质激素可能部分通过调节哮喘患者TH 亚群分泌细胞因子而在治疗中起作用。
Objective In order to observe the clinical effects of a new type of glucocorticoids methylprednisolone (MP) in the treatment of asthma, and explore the mechanism of MP in the treatment of this disease. Methods 30 asthmatic children were divided into two groups, one as MP treated group, and the other as control group. All blood samples were collected before and after treatment with MP. The serum IgE, cytokine production in the cultured supernatants of PBMC, and the cytokine mRNA expression were determined by ELISA and RT PCR methods respectively. Results (1) There were obvious clinical effects observed in the MP treated patients compared with the controls; (2) MP inhibited both T H1 and T H2 type cytokines; (3) MP inhibited both IFN γ and IL 4 mRNA expression. Conclusion The anti inflammatory action of MP in asthma may be associated with its immunomodulatory function, which influences the production of T H1 and T H2 type cytokines and blocks the cascade of inflammation.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2000年第6期548-550,共3页
Chinese Journal of Microbiology and Immunology
基金
阿斯特拉儿童哮喘研究基金资助项目
