摘要
以液料比、微波时间、微波功率和乙醇体积分数为自变量,绿原酸的收率为响应值,利用响应面分析法对杜仲叶中绿原酸的微波辅助提取工艺条件进行优化;并测试杜仲叶绿原酸提取物对自发性高血压大鼠的降压作用。结果表明:微波辅助提取杜仲叶中绿原酸的最佳工艺参数为杜仲叶粉碎颗粒度80目、液料比26:1(mL/g)、提取时间2.5min、微波功率722W、乙醇体积分数30%,在此条件下,绿原酸的提取得率达到29.74mg/g,与模型预测值吻合;该提取物的降血压实验结果显示,绿原酸提取物高剂量组(20mg/kg)对自发性高血压大鼠具有良好且稳定的降压效果。
Response surface methodology（RSM） was used to optimize the process conditions for microwave-assisted extraction of chlorogenic acid from Eucommia ulmoides leaves.Based on single-factor tests,a quadratic regression model was established to reveal the individual and cross-interaction effects of solid-liquid ratio,extraction time,microwave power and ethanol concentration on the yield of chlorogenic acid.The optimal extraction conditions were achieved to be powder size of 80 mesh,solid-liquid ratio of 1：26（g/mL）,extraction time of 2.5 min,microwave power of 722 W,and ethanol concentration of 30%.Under the optimal conditions,the actual yield of chlorogenic acid was 29.74 mg/g,which agreed with the theoretically predicted value.Meanwhile,the chlorogenic acid obtained in this study at a high dose（20 mg/kg） had a significant and stable antihypertensive effect in spontaneously hypertensive rats.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第14期30-34,共5页
Food Science
基金
国家自然科学基金青年科学基金项目(21101090)
江西省自然科学基金项目(2009GQY0190)
关键词
响应面法
微波辅助提取
杜仲叶
绿原酸
降压
response surface methodology
microwave-assisted extraction
Eucommia ulmoides leaf
chlorogenic acid
hypotensive effect
