摘要
目的探讨通塞脉方取舍怀牛膝对缺血性脑损伤模型大鼠的影响。方法雄性SD大鼠随机分为假手术组、模型组、尼莫地平(32.4 mg/kg)组、通塞脉全方(生药31.74 g/kg)组、去牛膝通塞脉方(生药27.77 g/kg)组。除假手术组与模型组外,其他给药组大鼠连续ig给药3 d,每天1次。给药后除假手术组外,其他组大鼠制备缺血性脑卒中模型,造模后24 h取血及大脑组织,脑组织以TTC染色,观察梗死率;HE染色观察病理学变化;测定血清中高迁移率组蛋白1(HMGB1)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的量;免疫组化法观察缺血组织中核因子-κB(NF-κB)的表达。结果模型组大鼠HMGB1、TNF-α、IL-1β的量及NF-κB的表达均明显升高(P<0.05),通塞脉全方及通塞脉去牛膝方均可降低脑缺血大鼠HMGB1、TNF-α、IL-1β的量,抑制NF-κB的表达(P<0.05),其中去牛膝通塞脉方降低IL-1β的作用最强。结论通塞脉全方及去牛膝通塞脉方均可通过抑制HMGB1相关的NF-κB信号通路的激活,发挥抑制炎症反应、减轻脑水肿、保护神经细胞的作用;去除牛膝的通塞脉方不会降低对脑缺血疗效,在抑制炎症因子IL-1β的分泌方面优于通塞脉方全方。
Objective To investigate the effects of Tongsaimai Prescription （TSMP） and TSMP without Achyranthis Bidentatae Radix （ABR） on ischemic brain injuries in rats. Methods The male SD rats were divided into Sham, model, Nimodipine （32.4 mg/kg）, TSMP （crude drug 31.74 g/kg）, and TSMP without ABR （crude drug 27.77 g/kg） groups. Except the Sham and model groups, the rats were ig administered once daily, for 3 d. After the treatment, except the Sham group, the cerebral ischemic stroke model was established. After 24 h, the blood and brain tissue were taken, the brain tissue was stained with TTC, and the cerebral infarction ratio was measured. The pathological changes were observed, and the contents of HMGB 1, TNF-α, and IL-1β in serum were detected. The expression of NF-κB in ischemia tissue was observed by immunohistochemisty method. Results The expression of HMGB1, TNF-ct, and IL-1β increased obviously （P 〈 0.05）. TSMP and TSMP without ABR could decrease the contents of HMGB1, TNF-α, and IL-1β, inhibit the expression of NF-κB （P 〈 0.05）, and the effect of TSMP without ABR on decreasing IL-1β was stronger. Conclusion Both TSMP and TSMP without ABR could inhibit the activation of HMGB 1-related NF-κB signal pathway with the function of anti-imflammation, alleviated encephaledema, and protecting nerve cells. TSMP without ABR has the similar effect on the ischemic brain injuries and the better effect on inhibiting the secretion of inflammatory factor IL- 1β.
出处
《中草药》
CAS
CSCD
北大核心
2013年第15期2130-2135,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81073071)
江苏省优势学科建设工程资助项目(2011ZYX3-0021)
关键词
通塞脉方
去牛膝通塞脉方
脑缺血
炎症因子
NF-ΚB信号通路
Tongsaimai Prescription
Tongsaimai Prescription without Achyranthis Bidentatae Radix
brain ischemia
inflammatoryfactor
NF-rJ3 signal pathway
