摘要
目的探讨雌激素在胃癌发生、发展中的作用及临床意义.方法应用流式细胞术检测31例胃癌组织、10例正常胃组织雌激素受体(estrogen receptor,ER)和细胞周期蛋白 D_1(cyclinD_1)表达量;应用免疫组织化学法检测31例胃肠癌组织、10例正常胃肠组织中 ER 和增殖细胞核抗原(proliferatingcell nuclear,PCNA)蛋白表达.结果流式细胞术定量检测结果表明,胃癌组织 ER 表达量(FI,1.19±0.23)明显高于正常胃组织(FI,1.00±0.04,P<0.01);胃癌组织 cyclinD_1表达量(FI,1.33±0.17)明显高于正常胃组织(FI,1.00±0.09,P<0.01).胃癌组织 ER 表达量与 cyclinD_1表达量存在正相关关系(r_s=0.40,P<0.05).免疫组织化学染色结果表明,胃癌组织 ER 表达阳性率为45%(14/31),明显高于正常胃组织0%(0/10,P<0.01);胃癌组织 PCNA 蛋白表达阳性率为97%(30/31),明显高于正常胃组织0%(0/10,P<0.01),且胃癌组织 ER 表达等级与 PCNA 蛋白表达等级间存在正相关关系(r_s=0.44,P<0.05).结论雌激素在胃癌发生、发展中有促进细胞增殖的作用,部分胃癌很有可能是雌激素依赖性肿瘤.
To investigate the role and clinical significance of estrogen in the pathogenesis of gastric carcinomas (GC). METHODS The estrogen receptor (ER) expression and the cyclinD1 expression were quantitatively determined by using flow cytometry (FCM) in 31 cases of GC and 10 cases of normal gastric tissues (NGT).The expressions of ER and proliferating cell nuclear antigen (PCNA) protein were examined immunohistochemically in 31 cases of GC and 10 cases of NGT. RESULTS FCM analysis showed that the ER expression in GC (FI,1.19±0.23) was significantly stronger than that in NGT (FI,1.00±0.04,P<0.01);The cyclinD1 expression in GC (FI,1.33±0.17) was also significantly stronger than that in NGT (FI,1.00±0.09,P<0.01).The results indicated that there was a significant positive correlation between the ER expression and the cyclinD1 expression in GC (r_s=0.40,P<0.05).Immunohistochemical staining results revealed that when compared with the positive rates of both ER and PCNA of NGT (0.00% wholly),the ER positive rate (45%,14/31) and the PCNA positive rate of GC (97%,30/31) were significantly higher (P<0.01). There was significantly positive correlation between the expression grading of ER and PCNA proteins (r_s=0.44, P<0.05). CONCLUSION Estrogen plays can accelerate cell proliferation in the occurrence and development of GC. There is a possibility that some GC are estrogen dependent tumors.
出处
《世界华人消化杂志》
CAS
2000年第10期1117-1120,共4页
World Chinese Journal of Digestology
关键词
胃肿瘤
雌激素类
增殖细胞核抗原
流式细胞术
stomach neoplasms
estrogens
proliferating cell nuclear antigen
cyclins
flow cytometry
immunohistochemistry
