摘要
目的探讨母婴分离导致大鼠成年后行为异常的早期存在机制。方法新生大鼠随机分为分离组和对照组,分离组于出生后第2至21天与母鼠每天分离3 h,对照组不做任何处理。在大鼠出生后第7、14、21天处死,取脑组织,以免疫组化检测大鼠海马组织小窝蛋白-1(Caveolin-1)、胶质纤维酸性蛋白(GFAP)、脑源性神经生长因子(BDNF)。以Image-Pro Plus图像分析系统对免疫组化图像进行半定量分析。结果与对照组相比,分离组大鼠出生后第7天Caveolin-l表达的差异无统计学意义,而GFAP和BDNF表达增加,差异有统计学意义(P<0.05);出生后第14和21天,分离组大鼠Caveolin-l、GFAP、BDNF表达下降,差异均有统计学意义(P<0.05)。结论新生期母婴分离可致大鼠海马Caveolin-l、BDNF、GFAP的表达出现不同程度的下降,这可能与母婴分离致成年后行为异常有关。
Objective To explore the potential mechanism of abnormal behavior resulted from maternal separation in neonatal period in rat.Methods Neonatal rats were equally and randomly divided into maternal separation group and control group.The rats in maternal separation group were separated from the dam for 3h per day on postnatal days（PND） 2 to 21,nothing was done to the rats in the control group.The brain tissues were taken out after being killed on PND 7,14,and 21.The expressions of Caveolin-l,BDNF and GFAP in hippocampal formation were detected by immunohistochemistry.Semiquantitative assessment of immunohistochemical images was performed by Image-Pro Plus software.Results Compared with control groups,the expression of Caveolin-l on PND 7 had no significant change,while BDNF and GFAP were significantly increased in maternal separation group（P＆lt;0.05）.On PND 14 and 21,the expressions of Caveolin-l,BDNF and GFAP were significantly decreased in maternal separation group（P＆lt;0.05）.Conclusions Decreased expressions of Caveolin-l,BDNF and GFAP caused by maternal separation in neonatal period may be associated with abnormal behaviors in adulthood in rat.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2013年第8期752-756,共5页
Journal of Clinical Pediatrics
基金
泸州医学院青年基金和泸州市科技局重点项目[No.2010-S-13(5/9)]
关键词
母婴分离
免疫组化
大鼠
maternal separation
immunohistochemistry
rat
