摘要
目的对吴茱萸的肝脏毒性提供证据。方法①肝脏毒性研究中,将ICR小鼠平分为四组,其中三组作为剂量组分别经口灌胃给予吴茱萸水煎剂40,60,80 g/kg体重,最后一组给予蒸馏水。所有动物均在24 h以后处死,并且测量谷丙转氨酶,谷草转氨酶,乳酸脱氢酶和总胆红素。同时观察肝脏的病理组织学变化。②将吴茱萸水煎剂用氯仿,正丁醇直接萃取分为三个极性部分并且准备了挥发油。所有组均是分别经口以120 g/kg给予以上萃取物。24 h后测定ALT。结果比较对照组,60 g/kg,80 g/kg组的小鼠的血清ALT,AST,LDH和T-Bil活性均明显升高(P<0.01)。病理组织学研究也表明了肝脏组织的变化。氯仿提取物受试组小鼠血清ALT有明显升高(P<0.05)。结论吴茱萸水煎剂有肝脏毒性作用;毒性成分存在于氯仿萃取部分。
Objective This study aims to provide evidence for hepatic toxicity. Methods （ 1 ） Male ICR mice were divided into four groups, of which three groups were treated with the decoction of Fructus Evodiae in a dose of 40,60,80 g/kg body weight respectively,the last were treated with distilled water. After 24 h, ALT, AST, LDH and T - bil were measured. Histopathological changes of liver were observed. （ 2 ） We divided the decoction of Fruetus Evodiae by chloroform, n - butyl alcohol into three extraction parts and prepared volatile oil extract. All groups were orally treated with above extracts in a dose of 120 g/kg body weight once. ALT was measured after 24h. Results Serum ALT, AST, LDH and T - Bil activities of 60g/kg, 80g/kg groups significantly increased than the vehicle control group （P 〈 0.01 ）. Histopathology studies also indicated a change of liver. The serum of chloroform extract group presented a significant increasing activity in ALT （ P 〈 0.05 ）. Conclusion It demonstrates that the decoction of Fructus Evodiae had hepatic toxic effect. Until now,we found the toxic ingredients lied in its chloroform extract fraction.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2013年第8期1810-1813,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家科技部科技基础性工作专项项目(No.2007FY230500)
关键词
吴茱萸
肝脏毒性
药物安全
氯仿萃取
Fructus Evodiae
Hepatic toxicity
Drug safety
Chloroform extract
