摘要
目的:研究木通皂苷D对Aβ诱导损伤的PC12细胞修复作用及相关基因表达水平的影响。方法:采用MTT法测定木通皂苷D对Aβ诱导损伤的PC12细胞修复作用,采用实时荧光定量PCR仪测定木通皂苷D对A2M、ECE2基因表达的影响。结果:在15μmol.L-1的浓度下,在6,12,24,48 h时间点,Aβ25-35诱导的PC12细胞损伤的抑制率与空白组相比,差异均有极显著性(P<0.01),且24 h时抑制率最高。木通皂苷D在不同给药时间逐渐表现出细胞增殖作用,且在作用16 h后产生明显的增殖效应,差异有极显著性(P<0.01);其中木通皂苷D处理24 h的细胞增殖作用最强,差异有极显著性(P<0.01)。与空白组比较,模型组中ECE2基因和A2M基因的相对表达量明显上调;木通皂苷D不同给药时间组PC12细胞中的ECE2基因和A2M基因的相对表达量与模型组相比明显下调。结论:木通皂苷D对Aβ诱导损伤的PC12细胞具有修复作用,其机制是通过下调ECE2基因和A2M基因的相对表达量修复Aβ25-35诱导的PC12细胞损伤。
OBJECTIVE To study Akebia saponin D repairing the damage of PC12 cell line by Aβ25-35 and its effects on A2M and ECE2 gene expression level. METHODS MTT was used to analyze the repairing effects of Akebia saponin D on PC12 cell line damaged by Aβ22-32 ; RT-qPCR was used to measure Akebia saponin D influencing on A2M and ECE2 genes expression lev- el. RESULTS At the concentration of Aβ25-35 15 μmol.L-1, the inhibition on PC12 cell line was significant after various treat ment time of 6 h, 12 h, 24 h, and 48 h（P〈0. 01）. After 16 h, Akebia saponin D could efficiently protect PC12 cells against Aβ25-35 induced cytotoxicity and prevent from the loss of cell viability, and it had the highest percentage of proliferation after 24 h （P〈0. 01 ）. Compared to control group, the expression levels of ECE2 and A2M mRNAs were strikingly higher in the modelgroup. ECE2 mRNA and A2M mRNA in PC12 cell treated with Akebia saponin D were obviously down regulated. CONCLUSION Akebia saponin D can repair the damage of PC12 cell line by Aβ25 -35, and it may be function through the down- regulation of genes ECE2 and A2M.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2013年第16期1304-1307,共4页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学重点项目(编号:30730113)
