摘要
目的观察二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对脂多糖(LPS)刺激的大鼠系膜细胞(GMCs)β1整合素(β1 integrin)和整合素连接激酶(integrin-linked kinase,ILK)表达的影响。方法用LPS(10mg/L)刺激大鼠GMCs,经EPA(10μmol/L、100μmol/L)、DHA(10μmol/L、100μmol/L)培养24 h、48 h后,采用实时定量PCR方法检测β1 integrin和ILK mRNA的表达。结果 LPS刺激可使GMCsβ1 integrin和ILK mRNA的表达增加;经EPA和DHA处理后,β1 integrin和ILK mRNA的表达下降。结论 EPA和DHA可以通过降低LPS诱导的GMCsβ1整合素和ILK的表达,对损伤的GMCs起保护作用。
Objective： To observe the effects of eicosapentaenoic acid（EPA） and docosahexaenoic acid（DHA） on lipopolysaccharide（LPS） induced expression of β1 integrin and integrin linked kinase（ILK）.Methods：The glomerular mesangial cells（GMCs） were stimulated by lipopolysaccharide（LPS） and incubated with EPA（10 μmol/L or 100 μmol/L） and DHA（10 μmol/L or 100 μmol/L） for 24 h or 48 h.The mRNA expressions of β1 integrin and ILK were detected by real-time PCR method.Results：LPS promoted β1 integrin and ILK mRNA expression,while EPA and DHA inhibited the GMCs β1 integrin and ILK mRNA expression induced by LPS.Conclusion：EPA and DHA might inhibit the GMCs β1 integrin and ILK mRNA expression induced by LPS,for preserving injury GMCs.
出处
《泰山医学院学报》
CAS
2013年第2期101-103,共3页
Journal of Taishan Medical College
