摘要
目的:探讨茶多酚(TP)对草甘膦诱导的小鼠睾丸支持细胞损伤的影响。方法:原代培养小鼠睾丸支持细胞,采用油红O染色鉴别支持细胞纯度。将细胞随机分为正常对照组、草甘膦染毒组(90μg/ml)、TP预处理组(经40、80、160μg/ml TP预处理12 h后,再用草甘膦作用于细胞24 h)。MTT法检测细胞存活率,比色法检测细胞乳酸脱氢酶(LDH)活性,TUNEL法原位检测细胞凋亡。结果:与对照组和TP预处理组比较,草甘膦染毒组能降低细胞存活率、增加LDH释放与细胞凋亡率(P<0.01);40、80μg/ml TP预处理组能明显提高支持细胞存活率,减少LDH释放量,降低支持细胞凋亡率(P<0.01);160μg/mlTP预处理组与草甘膦染毒组差异均无统计学意义(P>0.05)。结论:一定剂量的TP(40、80μg/ml)能保护由草甘膦诱导的小鼠睾丸支持细胞损伤。
Objective： To investigate the effect of tea polyphenols （TP） on glyphosate induced mouse sertoli cell（SC）injury. Methods ： The mouse sertoli cell was cultured and identified by oil red O. The cells were divided randomly into control group, glyphosate model group（ the SC was treated with glyphosate at 90 μg/ml） and TP pretreatment group （the SC was pretreated with 40, 80, 160 μg/ml TP for 12 hours, then treated with glyphosate for 24 hours, respectively）. The cell viability was measured by metylthiazdyhetrazolium method,lactate dehydrogenase （LDH） activity was determined by colorimetric method, and the cell apoptosis was observed by TUNEL method. Results： In contrast to control group, the cell survival rate was decreased, LDH activity and cell apoptosis ratio were increased in glyphosate model group（ P 〈 0.01 ）. In TP pretreatment at 40 μg/ml and 80μg/ml groups, the cell survival rate was significantly improved, LDH release and cell apoptosis ratio were decreased（ P 〈 0.01 ）. In TP pretreatment at 160 μg/ ml group, there were no significant changes than that in glyphosate model group （ P 〉 0. 05 ）. Conclusions： The appropriate concentrations of TP at 40 μg/ ml and 80 μg/ml can protect sertoli cell against glyphosate induced injury.
出处
《蚌埠医学院学报》
CAS
2013年第6期652-654,共3页
Journal of Bengbu Medical College
基金
安徽省教育厅自然科学研究资助项目(2006kj345B)
蚌埠医学院自然科学研究资助项目(BY1021)
蚌埠医学院自然科学研究资助项目(BY0835)
