摘要
目的探讨慢性消化性溃疡与胃癌患者血清DKK-1蛋白表达情况及其临床意义。方法选择2008年1月—2011年10月彭州市人民医院收治的慢性消化性溃疡43例,根据溃疡病变分为一般溃疡组27例,巨大溃疡组16例;选择同期胃大部切除术后病理确诊的胃癌38例作为胃癌组,体检健康者或无任何消化系统疾病者41例作为对照组。4组空腹采集静脉血后离心取上清液,采用酶联免疫吸附法检测DKK-1蛋白表达情况,分析血清DKK-1蛋白表达水平与胃癌TNM分期的相关性。结果对照组血清DKK-1蛋白表达水平与一般溃疡组比较差异无统计学意义(P>0.05),明显高于巨大溃疡组及胃癌组(P<0.05);一般溃疡组血清DKK-1蛋白表达水平明显高于巨大溃疡组(P<0.05);胃癌组血清DKK-1蛋白表达水平明显低于对照组、一般溃疡组和巨大溃疡组(P均<0.05)。不同TNM分期胃癌患者血清DKK-1蛋白表达量比较差异有统计学意义(F=31.23,P=0.000)。一般线性回归残差图显示,胃癌患者血清DKK-1蛋白表达量与TNM分期呈负相关(r=-5.32,P=0.001),且呈近似直线关系。结论巨大溃疡与胃癌患者的血清DKK-1蛋白表达均下调,DKK-1蛋白可能成为巨大溃疡或癌变的一种新的血清学诊断和病情监测指标。
Objective To explore the expression and clinical significance of serum Dickkopf-1（DKK-1） protein in patients with chronic peptic ulcers and gastric cancers.Methods During January 2008 and October 2011 43 patients with chronic peptic ulcers were divided into common ulcers group（group A,n =27）,and huge ulcers group（group B,n =16）;38 patients with gastric cancers confirmed by postoperative pathology underwent subtotal gastrectomy as gastric cancers group（group C）,and another 41 healthy people or without any digestive system diseases were chosen as control group.Enzyme-linked immuno sorbent assay（ELISA） was performed for detecting expressions of serum DKK-1 protein from empty stomach of venous blood in four groups by clear supernatant liquid after centrifugation.The correlation between expression of serum DKK-1 protein and TNM staging of gastric cancer was analyzed.Results The expression of serum DKK-1 protein in control group did not show statistically significant difference compared with that in group A（P 0.05）,while it was significantly higher than those in group B and C（P 0.05）;the expression of serum DKK-1 protein in group A was significantly higher than that in group B（P 0.05）;serum DKK-1 protein level in group C was significantly lower than those in control group,group A and B（P 0.05）,and the ulcer group（both the general and huge ulcer group）（average P 0.05）.The differences of DKK-1 protein expression in various TNM stages in patients with gastric cancers were statistically significant（F = 31.23,P = 0.000）.The common linear regression of residual plot showed that there was negative correlation between expression of serum DKK-1 protein and TNM staging in patients with gastric cancers（r =-5.32,P =0.001）,and it was nearly linear correlated.Conclusion The expression of serum DKK-1 protein is down regulation in patients with huge ulcers or gastric cancers,which may be a new biomarker of serological diagnosis and in monitoring pathogenetic conditions for the huge ulcers or gastric cancers.
出处
《临床误诊误治》
2013年第8期86-89,共4页
Clinical Misdiagnosis & Mistherapy
基金
国家"十一五"重大科技专项(2009zx1004-905)
关键词
DKK-1蛋白
溃疡
胃癌
酶联免疫吸附法
DKK-1 protein
Ulcer
Gastric cancer
Enzyme linked immunosorbent assay
