摘要
探讨中等强度抗阻力训练对骨骼肌PI3K基因和Akt基因表达的影响。方法:18只健康的SD大鼠随机分为安静组、有氧运动组和抗阻力训练组,每组6只。训练组采用Bedford等人跑台训练方案。8周训练结束后将大鼠安静状态下处死,取股四头肌,液氮冻存。采用Reverse Transcription-PCR方法测定大鼠股四头肌PI3K mRNA和AKt mRNA表达。结果:8周训练后,抗阻力运动组大鼠股四头肌PI3K mR-NA的表达较安静对照组和有氧运动组显著增多(p<0.05)。Akt mRNA表达较有氧运动组无显著差异(p>0.05),较安静对照组有显著增长(p<0.05)。结论:抗阻力跑台运动和有氧运动均可促进大鼠股四头肌PI3K mRNA和Akt mRNA表达,推测抗阻力运动可增强PI3K/Akt信号途径中信号转导,对促进糖原和蛋白合成有促进作用。
To explore the influence of the moderate intensity resistance training on the expression of PI3K and Akt gene in skeletal musle.Methods： 18 healthy Sprague-Dawlev rats were randomly distributed into three groups： normal control group（NC,n = 6）;aerobic exercise group（AE,n = 6）;medium resistance training group（MRT,n =6）.Quadriceps femoris muscles were excised after 8 weeks training and freezed in liquid nitrogen and analyzed by reverse transcription PCR.Results： After 8 weeks resistance training,the expression of PI3K mRNA in medium resistance training rats was significantly different from that in normal control group and aerobic exercise group rats（p 0.05）.And the expression of Akt mRNA has no significant difference between resistance training group and aerobic exercise group rats（p 0.05）,but medium resistance training group was markedly higher than normal control group in the expression of Akt mRNA（p 0.05）.Conclusion： The expression levels of PI3K mRNA and Akt mRNA in quadriceps femoris muscles can be improved by resistance training and aerobic exercise.It conjectures that treadmill resistance exercise can strengthen the signal transduction of PI3K / Akt signal pathway,resulting in promote glycogen and protein synthesis.
出处
《福建体育科技》
2013年第4期31-33,共3页
Fujian Sports Science and Technology
