摘要
【目的】探讨硫化氢（HzS）预处理对大鼠心肌缺血再灌注时孓腺苷蛋氨酸合成酶（SAM—s）表达的影响。【方法】健康成年孓D雄性大鼠50只，随机分成5组，每组10只。假手术组（S组）仅开胸并分离冠状动脉左前降支，但不阻断血流150min；缺血再灌注组（IR组）行冠状动脉左前降支阻断30min，再灌注120min；硫化氢1组（H1组）予以静脉注射硫氢化钠（NaHS）0．025mg／kg，给药后24h同IR组处理；H2S2组（H2组）予以静脉注射NariS0．05mg／kg，给药后24h同IR组处理；HzS3组（H3组）予以静脉注射NariS0．10mg／kg，给药后24h同IR组处理。再灌注结束后测心肌梗死面积，免疫印迹法测心肌SAM-s的表达。【结果】与IR组心肌梗死面积（38．27％±5．64％）比，各H2s组心肌梗死面积均减小（P〈0．05）。与S组比，IR组和H2S组SAM-s均升高（P〈0．05），与IR组比，各H2S组SAM—s表达均降低（P〈0．05）。【结论】H2S预处理对大鼠心肌损伤的保护作用可能与其抑制心肌SAM—s表达有关。
[Objective] To explore the effect of hydrogen sulfide preconditioning on the expression of S-adeno- sylmethionine synthetase during myocardial ischemia reperfusion in rats. [Methods] Totally 50 healthy SD male rats were randomly divided into 5 groups with 10 in each. Sham operation group（group S） underwent thoracotomy with separating left anterior descending coronary artery but without blocking blood flow for 150min. Ischemia reperfusion group（group IR） was given intravenous injection with Naris 0. 025mg/kg, and then the same treat- ment of IR group 24h later. Hydrogen sulfide 2 group（group H2） was given intravenous injection with Naris 0. 05mg/kg, and then the same treatment of IR group IR 24h later. Hydrogen sulfide 3 group（group H3） was given intravenous injection with Naris 0.10mg/kg, and then the same treatment of IR group 24h later. At the end of reperfusion, myocardial infarct size was measured, and the expression of SAM-s was detected by Western blot- ting. [Results] Compared with myocardial infarct size of IR group（38.27 % ＋ 5.64 %）, myocardial infarct size in other hydrogen sulfide groups was decreased（ P ~0.05）. Compared with group S, the expression of SAM-s in group IR and group H was increased（ P 〈0.05）. Compared with group IR, the expression of SAM- s in other hy- drogen sulfide groups was decreased（ P 〈0.05）. [Conclusion] Hydrogen sulfide preconditioning attenuates myo- cardial ischemia reperfusion injury possibly through down-regulating SAM-s expression in rats.
出处
《医学临床研究》
CAS
2013年第8期1475-1477,共3页
Journal of Clinical Research
基金
湖南省科技厅项目（2012TT2028）
关键词
再灌注损伤
心肌缺血
硫化氢
S-腺苷甲硫氨酸
大鼠
Reperfusion Injury
Myocardial Ischemia
Hydrogen Sulfide
S-Adenosylmethionine
Rats
