摘要
体内的稳态造血依赖于复杂而完整的骨髓造血微环境系统 ,其中的细胞成分是该系统的关键。存在于骨髓中的间充质干细胞 (MSCs)是成纤维细胞、内皮细胞、成骨细胞、脂肪细胞等多种骨髓基质细胞的前体细胞 ,在造血调控中可能具有一定的作用。本研究首先建立了成人骨髓MSCs的分离及体外培养的方法 ,并应用长期骨髓细胞培养体系 ,观察了MSCs滋养层体外维持长期培养启动细胞 (LTC IC)的能力及其促进造血细胞分化的功能。结果显示 :①脐带血来源的CD34+细胞粘附于滋养层上形成造血灶 ,表明MSCs可形成与骨髓基质细胞相似的体外造血微环境 ;②共培养 5周后造血细胞仍具有体外集落形成能力 ,说明MSCs具有维系LTC IC的能力 ;③流式细胞术分析显示 ,体外培养 5周后约 1%悬浮细胞表达CD34,15 %细胞CD4 1a阳性 ,提示MSCs促进造血细胞向巨核系细胞分化。
Bone marrow mesenchymal stem cells (MSCs) are multipotential progenitors of connective tissues and bone marrow stroma as well, which implies the modulatory function of MSCs in hematopoiesis. To clarify the contributions of MSCs to hematopoiesis, the methods for isolation and expansion of MSCs were established and long term bone marrow cultures were performed using irradiated MSCs as the feeder layer. The results here showed that CD34 + cells from cord blood formed hematopoietic foci adherent to the monolayer. Furthermore, colony forming cells remained in the coculture of 5 weeks, indicating the maintenance of long term culture initiating cells (LTC IC). Flow cytometry analysis showed that about 1% of the hematopoietic cells in the culture were positive for CD34 and around 15% were CD41a positive. It is clear that MSCs maintain LTC IC in vitro and promote differentiation of hematopoietic progenitors especially into megakaryocytic lineage. The preliminary results here demonstrate that MSCs residing in the bone marrow might be a crucial cellular component in the hematopoietic microenvironment.
出处
《中国实验血液学杂志》
CAS
CSCD
2000年第2期93-96,共4页
Journal of Experimental Hematology
关键词
间充质干细胞
骨髓
造血
造血微环境
造血调控
mesenchymal stem cell bone marrow hematopoiesis hematopoietic microenvironment hematopoietic modulation
