摘要
目的探讨胎盘间充质干细胞(PDMSCs)治疗新生大鼠低氧缺血性脑病(HIBD)的效果及其作用机制。方法HIBD模型乳鼠随机分为对照组、HIBD组、HIBD+PDMSCs治疗组和HIBD+成纤维细胞治疗组。细胞治疗后14 d评估各组生长发育及神经功能,用RT-PCR法和ELISA法检测海马和外周血中TNF-α、IL-17、IFN-γ和IL-10的变化。结果 HIBD组损伤侧大脑明显萎缩、神经行为严重障碍,发育较对照组明显落后(P<0.05),PDMSCs治疗后脑萎缩、神经行为障碍均改善,且幼鼠的体质量显著升高(P<0.05)。细胞治疗后14 d,HIBD组海马和外周血中TNF-α、IL-17和IFN-γ较对照组均升高,而IL-10水平降低(P<0.05);PDMSCs治疗组TNF-α、IL-17、IFN-γ较HIBD组有所降低,而IL-10水平升高(P<0.05)。结论 PDMSCs治疗能够改善新生HIBD大鼠的神经功能和发育,其作用机制可能是通过减轻机体的炎性反应。
Objective To investigate the neuroprotective effect of placenta-derived mesenchymal stem cells （PDMSCs） transplantation in rat＇s hypoxic-ischemic brain damage （HIBD） model and its mechanism. Methods The rats were randomly divided into four groups ： sham-operated group ; only HIBD group ; HIBD ＋ PDMSCs-treated group and HIBD ＋ Fibroblasts-treated group. After 2 weeks of treatment, we measured inflammatory cytokines （TNF-α, IFN-γ, IL-IO and IL-17） expression in the brain and serum by appropriate methods. Results Our results indicated that pro-inflammatory cytokine gene and protein expressions increased in the HIBD group, and that PDMSCs-treated group significantly （ all P 〈 0.05 ） lower. Furthermore, PDMSCs treatment was able to increase IL-10. We also observed that motor dysfunctions in fibroblasts-treated group were not improved and the indicators were not statistically significant compared with PDMSCs-treated group. Conclusions PDMSCs treatment plays a critical role in the neuroprotection observed in this model and the mechanism may be partly through alleviating inflammatory reaction.
出处
《基础医学与临床》
CSCD
北大核心
2013年第9期1186-1190,共5页
Basic and Clinical Medicine
关键词
间质干细胞
胎盘
低氧缺血
脑
炎性
mesenchymal stem cells
placenta
hypoxic-ischemic
brain
inflammatory reaction
