摘要
选择一株能产生麻痹性贝毒(paralytic shellfish poison,PSP)的赤潮甲藻塔玛亚历山大藻(ATHK株),研究其是否能通过引发中国明对虾的脂质过氧化作用而发挥其毒性作用。塔玛亚历山大藻粗提液经肌肉注射方式染毒中国明对虾,于染毒后1、3、6、12、24和48 h测定肝胰腺和鳃组织的超氧化物歧化酶(SOD),谷胱甘肽硫转移酶(GST)活性和丙二醛(MDA)含量。结果显示,染毒后1～6 h,中国明对虾肝胰腺和鳃组织SOD,GST活性均增加,12和48 h鳃组织的上述指标受到抑制。中国明对虾肝胰腺MDA含量除1 h外未见明显改变,鳃中MDA含量随时间增加呈升高趋势。研究表明,塔玛亚历山大藻粗提液对中国明对虾的鳃具有脂质过氧化作用,引起MDA含量增加,SOD和GST活性降低。
This study probes into effects of the toxic dinoflagellate Alexandrium tamarense （ATHK）, a producer of paralytic shellfish poison, on antioxidant system of Chinese shrimp Fenneropenaeus chinensis, an important mariculture species in China. The shrimp were intramuscularly injected the crude toxin extracted from A. tamarense cells. The dose injection was carried out only one time during the experiment, using extracted solution from 1.4 x 103 algae cells. Superoxide dismutase （SOD）activity, glutathione-S-transferase （GST） activity and malondialdehyde（MDA） content were analyzed in hepatopancreas and gill at 1,3,6,12, 24 and 48 h after stress. SOD activity and GST activity in hepatopancreas and gill increased within 6 h. However,they were inhibited in gill at 12 h and 48 h after stress. MDA content in hepatopancreas had no significant change except at 1 h after stress,but increased in gill with time prolonging. The results indicated that the crude toxin extracted from A. tamarense cells could cause lipid peroxidation in gill of F. chinensis by induction of MDA increase and SOD and GST inactivation.
出处
《水产学报》
CAS
CSCD
北大核心
2013年第8期1192-1197,共6页
Journal of Fisheries of China
基金
国家虾产业技术体系专项(CARS-47)
国家"八六三"高技术研究发展计划(2012AA10A409)
公益性行业(农业)科研专项(201103034)
