摘要
本研究旨在分析头孢噻呋在猪体内的残留消除规律,并为制定休药期提供依据。改进了猪可食性组织中头孢噻呋残留检测的高效液相色谱法(HPLC),试验猪按体重以5mg·kg-1肌注给药,连续给药3d,分别在最后一次给药后第12小时及第3、5、7、9天随机宰杀5头猪取样。样品先用二硫赤藓糖醇提取,再用碘乙酰胺衍生化后经MCX固相萃取小柱净化,经高效液相色谱C18柱分离后用紫外检测器检测。结果显示:给药后第12小时肾脏组织中的药物浓度最高,为2.589μg·g-1,第3天所有组织的药物浓度均低于最高残留限量(MRL)。采用Win-nonlin软件计算各组织的消除动力学参数,消除快慢依次为皮脂、肌肉、肝、肾和注射位点,其消除半衰期(t1/2β)分别为28.99、35.80、36.76、55.72和160.8h。肾的药时曲线下面积(AUC)最高,为123.4h·μg·g-1,说明肾脏是头孢噻呋作用的靶器官。参照EMEA对头孢噻呋制定的MRL,计算得注射位点的休药期最长,为98.64h。结果提示水性头孢噻呋注射液吸收迅速,体内分布广,消除较快,建议休药期为5d。
This experiment was conducted to study tissue residues of ceftiofur in swine,preparing for determination of the withdrawal time.A confirmative method has been improved to determine ceftiofur residues in edible tissues of pigs by high-performance liquid chromatography（HPLC）.Ceftiofur was administered to 30 healthy pigs intramuscularly at a dosage of 5 mg·kg-1 for 3 consecutive days.Animals were sacrificed at 12 hours and the 3rd,5th,7th,9th days after administration.The tissues were extracted with dithioerythritol,derivatized with iodoacetamide,and cleaned up on MCX solid-phase extraction cartridges.The analyte was detected by UV absorptive spectroscopy after separation by C18 column.The results showed that kidney had the highest drug concentration with 2.589 μg·g-1 at 12 hours,while the concentration of ceftiofur in all of tissues at 3 days was lower than the Maximum Residue Limit（MRL）.The pharmacokinetic parameters were estimated using Winnonlin software package.The results indicated that the elimination rate was skin/fat﹥muscle﹥liver﹥kidney﹥injection site,with the elimination half-life of 28.99,35.80,36.76,55.72,160.8 h respectively.Compared with other tissues,the area under concentration-time curve（AUC） in kidney was highest with 123.4 h·μg·g-1.Kidney was considered as the target organ.According to the MRL set by EU,the longest withdrawal time was suggested in injection site with 98.64 h.The results demonstrated that absorption and elimination was quickly and the distribution was wide.It is proposed that the withdrawal time was 5 days.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2013年第8期1311-1316,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
公益性行业(农业)科研专项(201303038-5)
关键词
高效液相色谱
头孢噻呋
残留
休药期
high-performance liquid chromatography
ceftiofur
residue
withdrawal time
