摘要
建立了一种高效液相色谱测定猕猴桃中维生素C含量的方法。采用0．1％草酸作为提取剂，色谱中采用HYPERSIL-C18色谱柱，0．1％草酸水作为流动相，流速为1．0mL／min，紫外检测器检测波长为254nm。结果表明，维生素C在10—500μg／mL内有良好的线性关系，线性回归方程为y=125．51x＋225．54，R^2=0．9987，方法回收率为79．2％～98．7％。该测定方法简便、快速，能准确测定猕猴桃中维生素C的含量。
A method of detecting the content of vitamin C in the kiwifi＇uit by HPLC was established. A HYPERSI L - C18 column was used,the flow phase was 0.1% oxalicacid, the flow rate was 1.0 mL/min and the detection wavelength was 254 nm in the HPLC system. The standard working curve of vitamin C had a good linear relation in the concentration range of 10 - 500 μ g/mL, the linear equation was y =125.51x ＋225.54 and the related coefficient was 0.998 7,the recoveries were 79.2%-98.7%. This method was simple,fast and suitable to detect the content of vitamin C in kiwifruit.
出处
《煤炭与化工》
CAS
2013年第4期140-141,共2页
Coal and Chemical Industry
