摘要
选择策勒黑羊BMPR-1B基因FecB突变的15只母羊,含3种基因型(基因型BB 6只,Bb 4只,bb 5只),摘取诱导发情24 h后的卵巢,提取总mRNA,采用Real-time PCR分析与卵泡发育相关的LHR、FSHR、PTGS2、GDF9、GDF5、BMP4和BMP15基因mRNA在3种基因型个体间的表达水平,以揭示策勒黑羊发生FecB突变后内在的分子作用机制。BMP4、BMP15、FSHR、LHR和PTGS2基因mRNA表达水平在FecB突变3种基因型个体间差异不显著(P>0.05);而GDF9基因mRNA在FecB突变纯合子与杂合子个体间的表达水平差异显著(P<0.05);GDF5基因mRNA在FecB突变纯合子与野生型个体间的表达水平差异显著(P<0.05),突变杂合子与野生型之间表达差异不显著,但3种基因型bb、Bb、BB个体的GDF5基因mRNA表达量呈依次下降趋势。以上结论说明,BMPR-1B基因突变后,GDF5基因的mRNA表达量下降,使得GDF5蛋白对卵母细胞颗粒细胞的抑制作用减弱,颗粒细胞分化和成熟比正常条件下要早,导致大量的卵母细胞发育成熟,表现为BMPR-1B基因FecB突变个体产羔数比野生型个体增加。
In order to explore the relationship between ovarian follicular developmental related gene and FecB mutation in Qira Black sheep, 15 ewes carrying FecB mutations in BMPR-1B gene including three genotypes （BB： 6, Bb： 4,bb： 5） were selected to collect ovaries after estrus induction for 24 h. Total mRNA expression levels of follicular developmental related genes including LHR, FSHR, PTGS2, GDF9, GDF5, BMP4 and BMP15 were detected by real-time quantitative PCR （RT-PCR）. The mRNA expression levels of BMP4, BMP15, FSHR, LHR and PTGS2 were not significantly different among the three genotypes （P〉0. 05）. The mRNA expression level of GDF9 gene was significantly different between the homozygote （BB） and heterozygote （Bb） （P〈0. 05 ）. GDF5 was significantly different between homozygote （BB） and wildtype （bb） （P〈0. 05）, and theexpression level decreased in turn from wildtype （bb）, heterozygote （Bb） to homozygote （BB）. It was suggested that the FecB mutation had decreased the expression level of GDF5 gene in some extent, resulting in inhibition decline of GDF5 protein against the growth of granulosa cells of sheep, which caused the maturity of more oocytes, and higher litter size as a result.
出处
《江苏农业学报》
CSCD
北大核心
2013年第4期807-811,共5页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(30760161)
关键词
策勒黑羊
FecB突变
卵泡发育
基因表达
Qira Black sheep
FecB mutation
ovarian follicular development
gene expression
