摘要
目的:建立脊痛宁胶囊中葛根素和芍药苷的含量测定方法。方法:采用高效液相色谱法,色谱柱为Diamonsil C18(2)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.5%磷酸溶液(13.5∶86.5),流速为1.0 mL.min-1,检测波长为230 nm,柱温为35℃。结果:葛根素在88.512～885.120μg.mL-1范围内线性关系良好(r=0.999 8),平均加样回收率为99.90%,RSD=1.28%(n=6);芍药苷在49.28～492.80μg.mL-1范围内线性关系良好(r=0.999 9),平均加样回收率为99.42%,RSD=1.02%(n=6)。结论:本方法简便、准确,专属性强,测定结果重复性好,是脊痛宁胶囊中葛根素和芍药苷同时定量分析的有效方法。
Objective： To establish the HPLC method for the simultaneous determination ofpuerarin and paeonifiorin in Jitongning capsule. Methods： HPLC was carried out on a Diamonsil C18column （250 mm × 4.6 mm, 5 μm）, the mobile phase consisted of acetonitrile-0.5% phosphoric acid solution （13.5 ： 86.5） at the flow rate of 1.0 mL.min~, the detection wavelength was 230 mn, and the column temperature was 35 ℃. Results： The linear range of puerarin was 88.512 - 885.120 μg·mL^-1（r = 0.999 8）. The average recovery was 99.90% with RSD of 1.28% （n = 6）. The linear range of paeoniflorin was 49.28 - 492.80 μg·mL^-1 （r = 0.999 9）, the average recovery was 99.42% with RSD of 1.02% （n = 6）. Conclusion： This method is simple and accurate, with good specificity and repeatability, and can be used for quantitative control of Jitongning capsule.
出处
《中国药物应用与监测》
CAS
2013年第4期200-202,共3页
Chinese Journal of Drug Application and Monitoring
