摘要
目的 寻找可用于诊断猪囊尾蚴病或可诱发宿主保护性免疫力的特异抗原。方法 用免疫血清筛选猪囊尾蚴头节 λZAP c DNA文库 ,阳性克隆基因测序 ,并亚克隆入表达载体 p ET2 8b。对重组体进行诱导表达并对表达产物进行 SDS- PAGE和 Western blot分析。结果 从 c DNA文库中筛到 2个阳性克隆 Cy1和 Cy4。 Cy4片段在原核体系 (p ET2 8b)中表达 ,获得高产量的融合蛋白Cy4。结论 Cy1为编码猪囊尾蚴抗原 B的片段 ;Cy4为一种新的猪囊尾蚴特异抗原 ,其作为诊断抗原或疫苗的潜力值得进一步研究。
Objective [WT5”BZ]To search for the gene encoding specific antigen as diagnostic antigen or vaccine candidate of cysticercosis. [WT5”HZ]Methods [WT5”BZ]A lambda ZAPⅡ cDNA library of [WT5”BX]Taenia solium [WT5”BZ]was screened with serum from the rabbit immunized with the cyst fluid. From the positive clone, Cy4 was subcloned into the expressive plasmid vector pET28b. The recombinants were induced to express the target protein Cy4,and it was analyzed by SDS-PAGE and Western blotting. [WT5”HZ]Results [WT5”BZ]Two positive clones Cy1 and Cy4 were obtained and sequenced. Cy4 was high expressed. The size of expressed protein Cy4 was about 42kDa with the same size as putative peptide together with the his-tag in the vector. The recombinant Cy4 could be recognized by serum from mouse immunized with the cyst fluid. [WT5”HZ]Conclusion [WT5”BZ]Cy1 is the partial sequence of paramyosin of [WT5”BX]Cysticercus cellulosae. [WT5”BZ]The probability of the recombinant protein Cy4 as a diagnostic antigen or vaccine needs to be further evaluated. [WT5”HZ]
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2000年第5期284-287,共4页
Chinese Journal of Schistosomiasis Control
