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培养条件对筛选根瘤菌突变体的影响 被引量:6

EFFECT OF CULTURE CONDITIONS ON SCREEN OF R.LEGUMINOSARUM MUTANTS
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摘要 将紫外线照射后的宁南2 号根瘤菌(Rhizobium legum inosarum Linglan 2)在不同光照和温度培养下表明,光照强度越大,照射时间越长,根瘤菌损伤就越大,存活率就越低,生长速度就越慢,而且光照还会降低突变株的耐酸碱特性。虽然培养温度对根瘤菌存活率和耐酸耐碱特性影响不大,但根瘤菌的生长和分裂速度则与温度有关。由此说明。 After ultraviolet irradiation R.Leguminosarum Linglan 2 is cultured in various irradiations and temperature, Study results show that companying irradiation intesity and time increase the injure degree of R. leguminosarum also increases, but its survival percent, growth rate and specific property of acid-alkaili tolerant decrease. Although effects of culture temperature are not very obvious on the specific property of R. leguminosarum in acid-alkaili tolerant, it is relation with the rate of R.leguminosarum growth and division. So, culture conditions are very important in the screen of R. leguminosarum mutants.
出处 《中国微生态学杂志》 CAS CSCD 1999年第6期346-348,共3页 Chinese Journal of Microecology
基金 四川省教委重点基金资肋项目
关键词 根瘤菌 突变体 培养条件 影响 R.leguminosarum\ Mutant\ Culture condition\ Effect
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