摘要
基因表达的变化有两种 ,即新出现的基因表达与表达量差异的基因表达 .表达量差异的基因克隆技术主要有mRNA差异展示 ,此技术是目前筛选差异表达基因最有效的方法之一 ,但主要存在假阳性率高的不足 ,针对此缺点 ,近几年提出了新的策略与方法 ,如差异消减展示、基于PCR和减法杂交基础上的差异表达基因克隆技术 ,这些技术具有显著优势 .
There are two kinds of changes of gene expression ,that is,novel gene expression and differential gene expression in quantity. Cloning technique of differentially expressed gene in quantity mainly is mRNA differential display, which is presently one of the most effective methods. However , mRNA differential display possesses higher unreal positive rate,in order to overcome its shortcoming, some novel strategies and methods were advocated ,such as differential subtraction display, subtraction based on LD PCR, LD PCR based on subtraction,those techniques have dominant advantages to mRNA differential display.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第4期362-364,共3页
Progress In Biochemistry and Biophysics
