摘要
天然产物抗氧化活性的评价是天然产物研究的重要组成部分,对其在日化领域的开发应用具有显著的指导意义。本文从实验原理、实验方法等方面对天然产物抗氧化活性的常见评价方法进行综述,详细介绍了化学分析法中的自由基(ABTS自由基阳离子、DPPH自由基、超氧自由基、羟自由基)清除实验、脂质过氧化抑制实验、还原能力测定及脂质氧化抑制等评价方法;描述了以生物细胞为模型的CAA抗氧评价方法;概述了在动物体内进行的抗氧化活性评价方法及指标,为相关领域中抗氧化天然产物的研究和开发提供实验理论基础。
Evaluation of antioxidant activity is important for the investigation of natural products, and is the useful guide for their application in chemical industry. In the present study, some typical analytical methods for evaluation of the antioxidant activity of natural products are reviewed in terms of their chemical mechanisms and common procedures. The in vitro methods, such as free radical （ABTS, DPPH, superoxide and hydroxyl free radical） scavenging, lipid peroxidation, reducing power and fat oxidation assays are introduced. Meanwhile, the cellular antioxidant activity （CAA） experiment based on determination of the vitality of cells is also described. Furthermore, the testing procedures and factors of in vivo method in animals are given. This review might be useful for the related research of natural products.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2013年第6期1205-1213,1247,共10页
Chemical Industry and Engineering Progress
基金
国家自然科学基金(21276165)
教育部博士点专项基金(20120181110078)项目
关键词
天然产物
抗氧化活性
评价方法
natural products
antioxidant activity
analytical method
