摘要
以啤酒酵母（Saccharomycescerevisiae)I菌株为出发菌株，经紫外线处理后，在含2.0mg/L的5-（2-氨基乙基）－L一半胱氨酸（AEC）的平板上获得18株生长良好的抗性突变林，编号为I－1～18，其中Ⅰ-Ⅱ株抗AEC达10～15mg／L，是出发株抗性最高浓度的10倍左右，并且Ⅰ—11株的L-赖氨酸含量（重量与重量比，100％）较出发株提高3.89％。以Ⅰ－11株作亚硝酸诱变处理，在含30～120mg／LAEC的培养基上又获得18株抗性突变株，其中Ⅰ11－L和Ⅰ11－MAEC的耐受浓度可达1300mg/L，其赖氨酸的含量较Ⅰ菌株的提高了27．42％和40．28％。
18 resistant mutants were collected after treating Saccharomyces cerevisiae strain Ⅰ as their starting strain with Liltraviolet. They grew well in ndneral medium (MM) containing 2. 0μg/ml 5 - (2 -andnoethyl) - L - cysteine (AEC)， in which strain Ⅰ could not grow. Ⅰ - 11, one of the mutants, could grow well in the MM containing 10 - 15μg/ml of AEC. About 10 times of maximum concentration that strain Ⅰ could resist. L - lysine in ferment broth of Ⅰ - 11 was 103. 98% of strain Ⅰ. Further treating Ⅰ -11with nitrite, another 18 strains were isolated from MM containing 30μg/ml of AEC. Two of thern, Ⅰ11 -L alld Ⅰ11 - M grew well in MM containing 1300μg/ml of AEC, and L - lysine conent in ferment juice of Ⅰ11 - L and Ⅰ11 - M were 127. 4 % and 140. 28% of strain I respectively.
出处
《微生物学杂志》
CAS
CSCD
1999年第4期15-16,21,共3页
Journal of Microbiology
关键词
啤酒酵母
结构类似物
赖氨酸
Saccharomyces cerevisiae
analogue
L-lysine
