摘要
目的研究N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对硅肺纤维化大鼠肺内胶原合成及核转录因子κB(NF-κB)p65的影响。方法将大鼠分为3组:对照组(每只大鼠支气管内灌注生理盐水1mL,8周后处死)、硅肺模型组(每只大鼠支气管内灌注SiO2混悬液1mL,8周后处死)、AcSDKP治疗组(每只大鼠支气管内灌注SiO2混悬液1mL,并持续给予AcSDKP 800μg·kg-1·d-1,8周后处死)。从HE染色、VG染色和羟脯氨酸胶原测定法检测各组大鼠肺纤维化程度及胶原含量,用免疫组化法和免疫印迹法检测NF-κB p65蛋白的表达。结果与对照组比较,硅肺模型组硅结节的数量、胶原含量和NF-κB p65的表达增高。与硅肺模型组相比,AcSDKP治疗组矽结节的数量、胶原含量和NF-κB p65的表达降低。结论 AcSDKP可能会通过增加B抑制蛋白(I-κB)的稳定性来抑制NF-κB p65的活性及表达,进而影响着肺泡炎与硅肺纤维化的进展。
Objective To investigate the effect of N-acetyl-seryl-aspartyl-lysyl-proline （AcSDKP） on the synthesis of collagen and the expression of NF-kB p65 in lung of rats with silicosis fibrosis. Methods Rats were divided into 3 groups randomly： the control group（each rat was intratracheally instilled with 1 niL normal saline and killed after 8 weeks,the silicotic model group（each rat was intratracheally instilled with 1 mL silica suspension and killed after 8 weeks,the AcSDKP treated group（each rat was intra- tracheally instilled with 1 mL silica suspension and AcSDKP（800 μg kg-1 d-1） was administered into every rat,then rats were killed after 8 weeks. Lung fibrosis in morphology and collagen content was observed by HE and vg staining and hydroxyproline as- say. The expression of NF-kB p65 was detected with the immunohistochemistry and Western blot. Results Compared with the con- trol group,the quantity of silicon nodules, collagen content and the expression of NF-kB p65were increased in the silicotic model group. Compared with the silicotic model group, the quantity of silicon nodules, collagen content and the expression of NF-kB p65 were decreased in the AcSDKP-treated group. Conclusion AcSDKP could inhibit the activity and expression of NF-kB p65 by strengthening the stability of I-kB,which may influence the progress of alveolar inflammation and silicosis fibrosis.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第26期3139-3141,共3页
Chongqing medicine
基金
邢台市科学技术研究与发展计划项目(2012ZC181)
